Fig. 2

Summary of changes in the incorporation level of SILAC amino acids into target protein. The labelling efficiency is calculated based on the XICs of the two peptides, VGIYFGMK and DPEFQAQFADLLK, using the formula: Incorporation % = [H/L]median/(1 + [H/L]median) ×100. The heavy/light (H/L) SILAC ratios are determined by analysing the ion chromatograms of the heavy and light peptides as they elute from a C18 column, and then calculating the ratio of the areas under XICs curves. The two reference peptides were detected in the tryptic digest of the recombinant enzyme extracted from 3 day-old biofilms pulsed with deuterated l-phenylalanine (row 1) or with deuterated l-lysine (row 4), 6 day-old biofilms chased with unlabelled phenylalanine (row 2) or with unlabelled lysine (row 5), and 9 day-old biofilms cross pulsed with deuterated l-lysine (row 3) or with deuterated l-phenylalanine (row 6)