Fig. 1

Metabolic pathway design for the production of 5-aminovalerate and glutarate in Corynebacterium glutamicum starting from the pathway precursor l-lysine. Heterologous genes (davBA) from Pseudomonas putida KT2440 were used to reconstruct the 5-aminovalerate pathway comprising lysine monooxygenase (DavB) and 5-aminovaleramidase (DavA). Genes under control of the constitutive tuf-promotor were integrated in the bioD locus of the genome of C. glutamicum LYS-12. Glutarate production from 5-aminovalerate via the activity of 5-aminovalerate transaminase (GabT) and glutarate semialdehyde dehydrogenase (GabD) relied on endogenous reaction with no or promiscuous gene annotation. Potential candidates were identified by BLASTX search using sequence information of the P. putida genes gabT (davT, PP_0213)^a and gabD (davD, PP_0214)^b