Table 2 Plasmid, strain or primer

Plasmid

 pRK2013

Km^R, mob⁺, tra⁺, helper plasmid

Laboratory stored

 pBBR1MCS5

Broad-host-range cloning vector; mod, rep, Gm ^R , lacZ

[21]

 pBBR1MCS5-P _tufB -ga2dh

pBBRMCS5 derivative expressing gox1230-gox1232 containing a tufB promoter, Gm ^R

This study

 pBBR1MCS5-P _ga2dh -ga2dh

pBBRMCS5 derivative expressing gox1230-gox1232 containing a ga2dh promoter, Gm ^R

This study

 pBBR1MCS5-P _gHp0169 -ga2dh

pBBRMCS5 derivative expressing gox1230-gox1232 containing a gHp0169 promoter, Gm ^R

This study

Strain

 E. coli DH5α

F-, ϕ80, lacZ∆M15, ∆(lacZYA-argF), U169, endA1, recA1, hsdR17(r_k-m_K+), supE44, λ⁻, thi-1, gyrA96, relA1, phoA

Tiangen, Beijing, China

 G. oxydans

Wild type, cef^R

DSM2003

 G. oxydans_pBBR1MCS5

G. oxydans contain plasmid pBBR1MCS5

This study

 G. oxydans_tufB_ga2dh

G. oxydans contain plasmid pBBR1MCS5-P _tufB -ga2dh, Cef^R, Gm^R

This study

 G. oxydans_g2adh_ga2dh

G. oxydans contain plasmid pBBR1MCS5-P _ga2dh -ga2dh, Cef^R, Gm^R

This study

 G. oxydans_gHp0169_ga2dh

G. oxydans contain plasmid pBBR1MCS5-P _gHp0169 -ga2dh, Cef^R, Gm^R

This study

Primer

 ga2dhf

CTATCTAGAGGAGAAACCTGTGCCCCCCATG

XbaI

 ga2dhr

GAGGGATCCTTCAGTTCAGTGAGACCGCATCATC

BamHI

 P _tufB _f

ACTGAGCTCCGATGGTAAGAAATCCACTGC

SacI

 P _tufB _r

ATATCTAGACCAAAACCCCGCTCCACC

XbaI

 P-ga2dh_f

CACTCTAGACAGAACCAGTGGCCGCCCCGACAAC

XbaI

 P-ga2dh_r

GAGCTGCAGTTCAGTTCAGTGAGACCGCATCATC

PstI

 P-gHp0169_f

ATAGAGCTCTGAAAGCGGCTGGCGCGT

SacI

 P-gHp0169_r

GCATCTAGAGCGGAAGGCGTTATACCCTGA

XbaI

rtPCR primers

 16s-RNA_f

GCGGTTGTTACAGTCAGATG

 16s-RNA_r

GCCTCAGCGTCAGTATCG

 gox1230_f

GCTGGAGCGAGGAAGACATC

 gox1230_r

CGGGTGGCAGACTTTTGAG

 gox1231_f

CCAGAACCTGTCCCAGTCCAC

 gox1231_r

CGACAACATTACGGCAAAGG

 gox1232_f

CGACAACATTACGGCAAAGG

 gox1232_r

GCCAGGGAAACCCAGCAT

 gox0265_f

AATGTTCGAGTGGGGTGGTC

 gox0265_r

CCCTTGGCGTCCTTTTCAG