Table 5 Plasmids used in this study as template for amplification of deletion markers and over-expressing of heterologous genes
Name | Details | Origin |
|---|---|---|
pUG74 | PCR template vector, source for loxP-flanked natMX gene deletion marker | [40] |
pCV3-ARO4 K229L | ARO4 K229L expression vector, source for ARO4 K229L–kanMX integration cassette | [8] |
pSP-G1 | Ura+ selectable double expression vector, contains TEF1–PGK1 bidirectional promoter | [39] |
pSP-G1-ABZ1 AWRI1631 -ABZ2 AWRI1631 ½ | pSP-G1 with ABZ1 and ABZ2 cloned under control of TEF1 and PGK1 promoter respectively | This study |
pSP-G1-ABZ1 AWRI1631 -ABZ2 AWRI1631 full | pSP-G1 with ABZ1 and ABZ2 cloned under control of TEF1 and PGK1 promoter respectively | This study |
pSP-G1-ABZ1 AWRI1631 -ABZ2 S288c | pSP-G1 with ABZ1 and ABZ2 cloned under control of TEF1 and PGK1 promoter respectively | This study |
pSP-G1-ABZ1 AWRI1631 -ABZ2 QA23 | pSP-G1 with ABZ1 and ABZ2 cloned under control of TEF1 and PGK1 promoter respectively | This study |
pSP-G1-ABZ1 QA23 -ABZ2 QA23 | pSP-G1 with ABZ1 and ABZ2 cloned under control of TEF1 and PGK1 promoter respectively | This study |