Table 4 Oligonucleotides used in this study
Name | Sequence (5′– >3′) |
|---|---|
MetJ-verify-f | TCTTTAGCAATCACCACG |
MetJ-verify-r | GGAATATTCTTGCCGTAAC |
PtsHICrr-verify-f | GAAAGGCGCAATCCAA |
PtsHICrr-verify-r | CGATTTGACTGCCAGAAT |
AroG*-f (BglII) | AAAAAAAGATCTGATGAATTATCAGAACGACGATTTAC |
AroG*-r (AvrII) | AAAAAACCTAGGCCTCCTTTAGATCCTTACCC |
AroG*-PpsA-TktA-f (BglII) | AAAAAAAGATCTGATGAATTATCAGAACGACGATTTAC |
AroG*-PpsA-TktA-r (AvrII) | AAAAAACCTAGGTTACAGCAGTTCTTTTGCTTTC |
MetK-f (NdeI) | AAAAAACATATGGCAAAACACCTTTTTAC |
MetK-r (AvrII) | AAAAAACCTAGGTTACTTCAGACCGGCAG |
Mtn-f (NcoI) | AAAAAACCATGGGCAAAATCGGCATCATTGG |
Mtn-r (NotI) | AAAAAAGCGGCCGCTTAGCCATGTGCAAGTTTCT |
LuxS-f (NotI) | AAAAAAGCGGCCGCTAATAAAGGAGATATACCATGCCGTTGTTAGATAGCTT |
LuxS-r (AflII) | AAAAAACTTAAGCTAGATGTGCAGTTCCTGC |