Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway

Table 1 Summary of titers from key experiments

Number	Figure	Host	Genes overexpressed	Methionine added (mM)	Protocatechuate (P) Titer (mg/L)	Protocatechuate (P) Titer (mM)	Vanillate (V) Titer (mg/L)	Vanillate (V) Titer (mM)	Cumulative P+V Titer (mM)
1	1B	PTS-glu + RARE’	aroG, asbF, OMT*	0	216	1.4	135	0.8	2.2
2	1B	PTS-glu + RARE’	aroG, asbF, OMT, ppsA, tktA*	0	308	2.0	101	0.6	2.6
3	3B	PTS-glu + RARE’	aroG, asbF, OMT, ppsA, tktA*	0	323	2.1	101	0.6	2.7
4	4D	RARE ∆metJ	aroG, asbF, OMT, ppsA, tktA*	0	262	1.7	202	1.2	2.9
5	4D	RARE ∆ *metJ*	**aroG, asbF, OMT, ppsA, tktA, metA, cysE***	0	185	1.2	269	1.6	2.8
6	5B	RARE ∆metJ	aroG, asbF, OMT, ppsA, tktA, sahH*	0	231	1.5	168	1.0	2.5
7	5C	RARE ∆ *metJ*	**aroG, asbF, OMT, ppsA, tktA, mtn, luxS***	0	277	1.8	252	1.5	3.3
8	3B	PTS-glu + RARE’	aroG, asbF, OMT, ppsA, tktA*	10	246	1.6	168	1.0	2.6
9*	4C	RARE ∆metJ	aroG, asbF, OMT, ppsA, tktA*	10	200	1.3	303	1.8	3.1
10	5D	RARE ∆metJ	aroG, asbF, OMT, ppsA, tktA, sahH*	10	154	1.0	269	1.6	2.6
11	5E	RARE ∆ *metJ*	**aroG, asbF, OMT, ppsA, tktA, mtn, luxS***	10	123	0.8	370	2.2	3.0
12	S3	RARE ∆ *metJ*	**aroG, asbF, OMT, ppsA, tktA, metA, cysE***	20	139	0.9	420	2.5	3.4

Rows in bold formatting are highlighted in “Discussion” section
* Titers shown for experiment 9 correspond to samples obtained 48 h after induction, which was the final sampling time. All other titers correspond to samples obtained 72 h after induction

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