Fig. 5

Effects of the trkH80, rpoS214 and lapB43 mutations on the viability of strains incubated in mixed culture. The strains tested as a minority (Kan^r) were grown as monocultures in Pi-limiting medium for 1 day, diluted 10⁵-fold into 50 ml of 1-day-old cultures of the ancestral strain ENZ1797 (ENZ535 Tc^r) in Pi-limiting medium, and incubated further for 8 days. On day 9 of incubation, 1 ml of mixed culture was added into 50 ml of fresh Pi-limiting medium and incubated further. At the end of the incubation period, the pH and the concentrations of glucose (Glc) and of acetate (Ace) were determined in spent media (concentrations are indicated in mM; the sign < indicates that the concentration was ≤0.02 mM). The viability of the ancestral strain (Tc^r) is indicated with dashed lines of different colours for independent experiments. The viability of the test strains (Kan^r) -ENZ2000 (a), ENZ1791 and ENZ2166 (b), ENZ2067 (c), ENZ2136 (d), ENZ2395 (e), ENZ2163 (f), ENZ2137 (g), and ENZ2003 and ENZ1984 (h)- is indicated with solid lines