Table 1 Primer sequences with restriction site underlined
Primer | Sequence |
|---|---|
Ppdc KpnI F | 5′-CGG GGTACCTTACGCTCATGATCGC-3′ |
Ppdc XhoI R | 5′-CCG CTCGAG TGCTTACTCCATATAT-3′ |
1623 XhoI F | 5′-CCG CTCGAG ATGGCAGAGACGACTACGG-3′ |
1623 XbaI R | 5′-TGCTCTAGACTAGTGGTCGAGGAAGCTCC-3′ |
Tpdc XbaI F | 5′-GACGGCTCTAGATAGTTTTTAAATAAACTTAGAGCTTAAG-3′ |
Tpdc SacI R | 5′-GCCTCGAGCTCAATTTTATAGAAAAGAAAAACAAAGC-3′ |
ZMO1360 F | 5′-TACAACCTCGTCCTTCTT-3′ |
ZMO1360 R | 5′-CATAACCTTCTGCACTGA-3′ |
ZMO1596 F | 5′-GGTATTAATTCTGCTGTT-3′ |
ZMO1596 R | 5′-CGAAGTCTGAATTGTTAT-3′ |
16s F | 5′-TCAACTATAGACCAGTAAGT-3′ |
16s R | 5′-AGAACATAGAAGAGGTAAGT-3′ |