Fig. 3

Secondary structure analysis of PrPs IBs by ATR-FTIR. The secondary structure content of PrP^WT (a) and PrP⁹⁰⁻²³¹ (b) inside the IBs was determined following ATR-FTIR absorbance of dry samples in the amide I region of the infrared spectrum (solid black line). Spectral components in the Fourier deconvoluted FTIR spectra (dashed gray line) are shown as solid gray lines (bands 1–5). The area and position of the correspondent bands are indicated in Table 1