Fig. 2

Isolation of PrP^WT and PrP⁹⁰⁻²³¹ IBs from E. coli cells. SDS-PAGE analysis of purified PrPs IBs. The band at ~16 kDa corresponds to the C-terminal region of PrP (PrP⁹⁰⁻²³¹) and that at ~25 kDa to the PrP^WT (rPrP²³⁻²³¹), both with the histidine-tag. The purification of both PrP monomers from their respective isolated IBs is also shown, as well as the remaining soluble proteins present in the supernatant after cell fractionation (SN). Molecular weight markers are shown on the left (MW)