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Table 2 MRM transitions used to quantify alkaloids in LC–MS/MS analysis

From: Optimization of yeast-based production of medicinal protoberberine alkaloids

Compound Quantifier MRM transition Qualifier MRM transition
Precursor → product ion Fragmentor Collision energy Precursor → product ion Fragmentor Collision energy
Reticuline 330 → 192 120 19 330 → 137 120 31
Scoulerine 328 → 151 135 30 328 → 178 135 29
Tetrahydrocolumbamine 342 → 178 135 29 342 → 163 135 29
Canadine 340 → 176 135 29 340 → 149 135 25
Berberine 336 → 320 135 29 336 → 292 135 25