Table 2 MRM transitions used to quantify alkaloids in LC–MS/MS analysis
From: Optimization of yeast-based production of medicinal protoberberine alkaloids
Compound | Quantifier MRM transition | Qualifier MRM transition | ||||
|---|---|---|---|---|---|---|
Precursor → product ion | Fragmentor | Collision energy | Precursor → product ion | Fragmentor | Collision energy | |
Reticuline | 330 → 192 | 120 | 19 | 330 → 137 | 120 | 31 |
Scoulerine | 328 → 151 | 135 | 30 | 328 → 178 | 135 | 29 |
Tetrahydrocolumbamine | 342 → 178 | 135 | 29 | 342 → 163 | 135 | 29 |
Canadine | 340 → 176 | 135 | 29 | 340 → 149 | 135 | 25 |
Berberine | 336 → 320 | 135 | 29 | 336 → 292 | 135 | 25 |