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Table 1 Recombinant antibody production: hot topics and examples

From: Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

Antibody applications

Opportunity and challenges

Achievement reports (references)

Protein delivery

Fusions of Ab fragments with active proteins (IL1, toxins, chromophores,…)

[4, 5, 12, 16, 87]

BiTE

Bispecific T-cell engager

[29]

Bispecific activity

Possibility to interfere or to join two different pathways

[30]

Cell display

Purification-independent methods for inexpensive Ab production

[31, 33, 47, 48, 97]

Viral nanoparticles

Development of self-assembling multivalent structures

[56]

In vivo expression and activity

Production of pathogen-neutralizing Abs directly in food and animals

[88, 90, 91, 93, 95]

Immunomodulation

Effective glycosylation for precise targeting and CDC/ADCC

[28, 76, 85, 86]

Cargo delivery

Ab chemical functionalization for ADC, radiotherapy, and imaging

[57]

Bacterial delivery

Immune-response and accumulation of active molecules

[45, 46]

Intrabodies

Necessity to fold into active form in cell reducing cytoplasm

[100, 105, 106]

  1. Recombinant antibodies are currently used in multiple applications which require the production of large amounts of qualitatively well-defined reagents that need as few as possible post-purification steps before being ready for the final application. Major biotechnological needs and representative examples of meaningful strategies for their accomplishment are listed below
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Microbial Cell Factories

ISSN: 1475-2859

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