- Open Access
Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes in Myxococcus xanthus
© Zhu et al. 2015
- Received: 17 May 2015
- Accepted: 7 July 2015
- Published: 22 July 2015
Exotic genes, especially clustered multiple-genes for a complex pathway, are normally integrated into chromosome for heterologous expression. The influences of insertion sites on heterologous expression and allotropic expressions of exotic genes on host remain mostly unclear.
We compared the integration and expression efficiencies of single and multiple exotic genes that were inserted into Myxococcus xanthus genome by transposition and attB-site-directed recombination. While the site-directed integration had a rather stable chloramphenicol acetyl transferase (CAT) activity, the transposition produced varied CAT enzyme activities. We attempted to integrate the 56-kb gene cluster for the biosynthesis of antitumor polyketides epothilones into M. xanthus genome by site-direction but failed, which was determined to be due to the insertion size limitation at the attB site. The transposition technique produced many recombinants with varied production capabilities of epothilones, which, however, were not paralleled to the transcriptional characteristics of the local sites where the genes were integrated. Comparative transcriptomics analysis demonstrated that the allopatric integrations caused selective changes of host transcriptomes, leading to varied expressions of epothilone genes in different mutants.
With the increase of insertion fragment size, transposition is a more practicable integration method for the expression of exotic genes. Allopatric integrations selectively change host transcriptomes, which lead to varied expression efficiencies of exotic genes.
- Site-directed integration
- Chloramphenicol acetyl transferase
- Epothilone biosynthetic gene cluster
- Expression efficiency
- Myxococcus xanthus
Heterologous expression is a routine laboratory technique to obtain massive quantities of interesting products, not only single recombinant proteins but also metabolites from complex biosynthetic pathways, such as microbial secondary metabolites. Efficient expression and high yields of exotic genes in acclimatized hosts are not only important for increasing the yields of desired products, but also useful in the discovery of novel compounds, for example, the products of those cryptic secondary metabolic pathways [1, 2]. Heterologous expressions of single gene products can normally be achieved in high efficiency and yields in Escherichia coli after codon optimization, promoter selection, co-expression with chaperon protein genes, and/or cultivation improvement [1–3]. However, heterologous expressions of metabolites from pathways containing cluster-organized multiple-genes confront much more difficulties and limits . Researchers have to establish suitable transfer systems for large-sized gene clusters, maintain integrity of well-organized genes during transfer, trigger efficient expressions of multiple genes, provide sufficient supplies of substrates for the biosynthesis, facilitate secretion to eliminate products-feedback inhibition on biosynthesis or toxic effects on host, and reduce negative effects of local metabolisms on the desired expressions. Up to now, efficient expression of a complicated biosynthetic pathway in heterologous host is still a challenge.
Because of large size, multiple genes of complicated biosynthetic pathways are often integrated into chromosome for heterologous expression. For example, antitumor compounds epothilones were originally isolated from myxobacterial Sorangium cellulosum cultures . The 56-kb biosynthetic gene cluster has been successfully introduced into different hosts, including Streptomyces coelicolor [6, 7], S. venezuelae , E. coli , Pseudomonas putida  and other myxobacterium Myxococcus xanthus [10–12]. While the yields of epothilones in M. xanthus might be up to 160 μg/L-titer , the yields in those distantly related hosts were lower than 1 μg/L, or undetectable [9, 10], probably due to incompatibility of the exotic DNA and/or metabolites. Thus, although distantly related hosts may have merits in genetic performances, growth and fermentation, closely related species turn to be preferable for heterologous expression of the products from large gene clusters. However, the productions of epothilones are also greatly varied in those engineered Myxococcus strains, even with almost identical genetic backgrounds [10, 11], suggesting internal uncertainty for the expression of multiple exotic genes. For example, it is yet unclear whether and how insertion sites influence the expression efficiency of exotic genes and whether and how allotropic expressions influence on host cells.
Transposition is able to bring exotic genes into host chromosome randomly. Recently, transposon technique has been developed for heterologous expression of large-sized gene clusters , which provides an approach for analysis of the influences of integration patterns. In this study, we compared expression efficiencies of the chloramphenicol acetyl transferase gene that was introduced into chromosome via transposition insertion or site-specific insertion. We then constructed vectors containing the entire 56-kb epothilone gene cluster, stitched using a modified recombination strategy, for one-step introduction into M. xanthus cells. We assayed effects of integration sites on the yields of epothilones and transcriptome changes in different mutants. Our results indicated that allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes in M. xanthus.
Site-specific and transposition insertions of cat gene in M. xanthus
There are usually two ways to introduce exotic genes into M. xanthus genome. One is arbitrary insertion via transposon such as miniHimar1, a plasmid derived from the mariner element Himar1 [13, 14], while the other is site-specific recombination, normally at the chromosomal attB site via Mx8 att, developed from myxophage Mx8 DNA [15, 16]. To test whether insertion pattern influences heterologous expression, we introduced the chloramphenicol acetyl transferase (cat) gene into the genome of M. xanthus DZ2 via either transposition (Tp) or site-directed insertion (Mx8). Previous studies related to heterologous expressions of the epothilone biosynthetic gene cluster normally employed an exotic promoter like aphII, the kanamycin promoter in pKK-aphII , which is frequently used in M. xanthus [18, 19]. We once reported that the 843-bp epoP promoter for the biosynthetic gene cluster of epothilones from S. cellulosum So0157-2 exhibited much higher activities than aphII in E. coli . Thus, four plasmids containing the respective promoter and integration elements, i.e. pTp-epoP, pMx8-epoP, pTp-aph and pMx8-aph, were constructed (Additional file 1: Figure S1), which were further electroporated into the M. xanthus DZ2 strain, respectively.
Construction of plasmids containing the entire epothilone biosynthetic gene cluster
Epothilones are biosynthesized by a gene cluster of seven multifunctional modules, spanning approximately 56 kb in length [20, 21]. In this study, we constructed the whole epothilone biosynthetic genes into single plasmids for the integration in M. xanthus chromosome. The epothilone genes were from the Cosmid10 and Fosmid3B11 plasmids, which separately contained a 38.5-kb fragment from epoA to the front part of epoD and a 34.4-kb fragment from the rear part of epoC to the downstream fragment of epoF. The two fragments overlapped a 6.5-kb region.
We also constructed a recombinant plasmid containing the Mx8 att sequence (Figure 2a, b) for site-specific recombination.
Integration of the epothilone biosynthetic genes into M. xanthus chromosome
The plasmids were subject to one-step integration into M. xanthus DZ2 chromosome, and the recombinant colonies were screened by selection markers Apra and Cm. After 6–8 days of incubation at 30°C, we obtained 21 colonies from the CYE plate plus the Apra and Cm antibiotics in a single transformation performance. PCR amplification determined that 14 of the 21 antibiotics-resistant colonies were correct transformants, while the other seven contained partial or none of epothilone genes. A further transformation performance of p15A-Tp-CF in M. xanthus DK1622 strain yielded 30 correct transformants from 59 antibiotics-resistant colonies. We also performed transformations in SW504, a difA mutant of M. xanthus DK1622 [22, 23], from which 4 correct transformants were obtained. Figure 2c demonstrates some PCR amplification results. Our results showed that the transposition technique was highly efficient to nail large exotic genetic fragments into the chromosome of different M. xanthus strains.
Electroporation of the p15A-Mx8-CF plasmid with M. xanthus DZ2 strain obtained six tiny colonies on the selection plate, which, however, were turned out to be spontaneous mutants without any epothilone genes. We performed transformation of the plasmid in different M. xanthus strains, which also failed in obtaining any correct transformant. To testify whether the failure was due to the size limitation for integration at the Mx8 att site, we constructed smaller plasmids of 42.0-kb p15A-Mx8-fos, derived from the recombination of the linear fosmid3B11 with p15A-recMx8, and 18.1-kb p15A-recMx8. The transformation performance with these two plasmids under the same conditions produced 4 and 47 correct colonies in M. xanthus DZ2, respectively. We thus concluded that the recombination efficiency of the Mx8 att site decreased sharply with the increase of the integration fragment sizes.
Epothilone production abilities and integration sites in the transposition transformants
Transcriptomics variation in epothilone-producing transposition transformants
Comparative transcriptomes showed similar distributions of expression density of the total genes in the four recombinants (Figure 5b). The expression patterns of the epothilone genes varied significantly in the three recombinants (Figure 5c), but parallel to their production abilities. The expression levels of the epothilone biosynthetic genes in ZE-5 were low, especially the latter part of this big gene cluster. In contrast, the epothilone genes expressions in ZE-9, which had approximately five times of the epothilone production in ZE-5, were rather high in different regions in the gene cluster. In the ZE-14 strain, although expressions of the front region were much high, the hinder genes had sharply decreased expressions, which probably led to the medium level of the epothilone production.
Compared to the M. xanthus DZ2 strain, many Myxococcus-own genes were up- or down-regulated distinctly in these allopatric insertion mutants (Additional file 4: Table S2). For example, three hypothetical protein genes (MXAN_5126, MXAN_5530 and MXAN_3967) were up regulated, while two genes (MXAN_4480 predicted for a DNA binding protein and MXAN_4324 for a hypothetical protein) were down regulated in the ZE-9 and ZE-14 strains. Six genes were specifically down regulated of their expressions in the ZE-9 strain, but were not significantly changed in ZE-5 or ZE-14. In all the three tested recombinant strains, three Myxococcus genes, i.e. MXAN_1576 (predicted encoding a major facilitator family transporter), MXAN_1093 (a DNA-binding response regulator) and MXAN_7163 (bis (5′-nucleosyl)-tetraphosphatase, symmetrical), were found to be significantly up-regulated, while two genes, i.e. MXAN_7372 (hypothetical protein) and MXAN_4372 (DNA-binding protein) were significantly down regulated. It is noted that the locations of these up- or down- regulated genes normally had no relationship with the insertion sites in Myxococcus genome. These transcriptome changes indicated that allopatric integration of exotic genes in genome had some specific effects on the transcriptome in host, which probably resulted in distinct expressions of the exotic genes.
In contrary to potential size-limitation of the inserted fragments at directed sites, demonstrated using the attB site in M. xanthus, transposition attempts to position large-sized genetic materials randomly into genome, thus provides much more opportunities for the integration of exotic genes. Such random transposition integration is blind, and the introduced exotic genes may inactivate or disturb functions of host genes, but also yields varied expression levels of exotic genes. The expression differences of allopatrically integrated genes did not follow the local expression characteristics, but rather the results of some global changes of the host transcriptome. We might be able to improve the expressions of the exotic genes further by genome engineering based on the transcriptome changes. In addition, the expressions of epothilone genes were rather different in different cluster regions–increasing the expression of low-expressed genes will be also useful for the yield improvement.
Bacterial strains, plasmids and culture conditions
Bacterial strains and plasmids used in this study
Strain and plasmid
Source or reference
F−, supE44, ΔlacU169 (φ80lacZΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1
An E. coli DH10B derive by deletion of fhuA, ybcC and recET, and insertion of the PBAD-gbaA cassette at the ybcC locus
Epothilone producing strain
dif mutant, contains a difA in frame deletion
p15A ori; Cmr
With an 843-bp epothione promoter epop inserted in front of the promoter-less cat gene (epop-cat); Cmr
With an aphII promoter fragment inserted in front of the promoter-less cat gene (aphII-cat); Cmr
Himar1 transposon (IR-Tpase-IR), lacZ; Kmr
Site specific integration vector with Mx8 attB
integration site (Mx8); Tetr
Ligating the epop-cat to the p15A ori; Cmr
Ligating the aphII-cat to the p15A ori; Cmr
Ligating the IR-Tpase-IR to p15A-epop-cat; Cmr
Ligating the IR-Tpase-IR to p15A-aph-cat; Cmr
Ligating Mx8 to p15A-epop-cat; Cmr
Ligating Mx8 to p15A-aph-cat; Cmr
A plasmid from a Cosmid library of So0157-2 genome, containing foremost part of epothilone gene cluster (epoA, epoP, epoB, epoC and part of epoD); Ampr, Neor
A plasmid from a fosmid library of So0157-2 genome, containing latter part of epothilone gene cluster (partial epoC, epoD, epoE, epoF); Cmr
Cloning vector; Ampr
Ligating the 2.0-kb epoup fragment, 2.2-kb epoCov fragment, and the 2.2-kb epodown fragment into pSL1180; Ampr
Gene replacement vector with KG cassette; Kmr
Cloning vector, Ampr
Ligating the 1.7-kb galk gene to pGEM-Teasy; Ampr
Ligating the 3.2-kb TG cassette to pSL-UCD; Ampr, Tetr
A suicide plasmid; Ampr
Ligating the 1.8-kb sacB gene to pSL-ETG; Ampr, Tetr
Conjunction Vector; Aprar
Ligating 1.7-kb apra fragment to p15A-aph-cat; Aprar, Cmr
Ligating IR-Tpase-IR to p15A-apra-cm; Aprar, Cmr
Ligating Mx8 to p15A-apra-cm; Aprar, Cmr
Ligating the 11.5-kb MunI-SpeI fragment from pSL-ETGS to p15A-apra-cm-Tp; Aprar, Cmr, Tetr
Ligating the 11.5-kb MunI-SpeI fragment from pSL-ETGS to p15A-apra-cm-Mx8; Aprar, Cmr, Tetr
Recombination between the linearized Fosmid3B11 and p15A-recTp; Aprar, Cmr, Tetr
Recombination between the linearized Fosmid3B11 and p15A-recMx8; Aprar, Cmr, Tetr
Recombination between the linearized Cosmid10 and p15A-TP-fos; Aprar, Cmr
Recombination between the linearized Cosmid10 and p15A-TP-fos; Aprar, Cmr
Construction of CAT reporter vectors
We amplified the 836-bp replicon p15A from pACYC184 using the primer pair of P15A. The chloramphenicol acetyl transferase (cat) gene was initiated by a promoter of either the epothilone operon promoter (epop) or the kanamycin promoter (aphII). These two primers are recognizable in E. coli and M. xanthus [10, 11, 17]. The 1.6-kb NdeI-KpnI fragment epop-cat was amplified from the pKK843 plasmid  using the CM-EPOP primer pair, while the 1.1-kb NdeI-KpnI fragment aphII-cat was amplified from pKK-aphII  using the CM-APH primer pair. The epop-cat and aphII-cat were ligated to the p15A replicon, generating the vectors of p15A-epop-cat and p15A-aph-cat, respectively. The 2.0-kb transposon element IR-Tpase-IR was amplified from plasmid pMiniHimar-lacZ  using the TPASE prime pair, while the 3.0-kb site-specific recombination element Mx8 was generated from the template pSWU30  using the MX8 primer pair. Finally, the plasmids pTp-epop (or pTp-aph) and pMx8-epop (or pMx8-aph) were constructed by ligating the IR-Tpase-IR and Mx8 to p15A-epop-cat (or p15A-aph-cat), respectively, which had been digested with NdeI; and the DNA ends were blunted using the T4 DNA polymerase. The PCR primer pairs used in this study are summarized in Additional file 5: Table S3.
Stitching and recombination of epothilone gene cluster
We constructed cosmid and fosmid libraries of Sorangium cellulosum So0157-2 genome. Southern blotting hybridization with epothilone gene probes revealed clones containing the epothilone genes. The harbored epothilone genes fragments in the positive clones were determined by the end sequencing. We selected Cosmid10 and Fosmid3B11 for our construction. After completely sequencing, Cosmid10 contained a fragment of complete epoA to epoC and part of epoD, while Fosmid3B11 contained part of epoC till the downstream sequence of epoF.
To stitch the two epothilone-genes fragments in Cosmid10 and Fosmid3B11, transitional recombinant vectors p15A- recT and p15A-recM were constructed as follows. First, the 2.0-kb BglII-XbaI epoup fragment (upstream of epoA), 2.2-kbXbaI-NdeI epoCov fragment (overlapping the epoC sequence), and the 2.2-kb NotI-SpeI epodown fragment (downstream of epoF) were amplified from the genomic DNA of S. cellulosum So0157-2 using the primer pairs of UP, C-LAP and DOWN, respectively. The three PCR products, used as the homologous arms for recombination, were ligated serially into the corresponding restriction sites of pSL1180, generating pSL-UCD. Then, the 1.7-kb galk gene, containing XbaI and NheI sites at the 5′ end and XbaI site at the 3′ end, was amplified from pBJ113  using the GALK primer pair, which was further ligated into the XbaI site of pGEM-Teasy vector, generating pGEM-galk. The 1.5-kb NheI tet fragment (tetracycline gene), amplified from pSWU30 using the TET primer pair, was ligated into the NheI site of the galk fragment in pGEM-galk. The 3.2-kb tet-galk fragment (TG cassette), used for positive and negative selection, was cleaved with Xba I, and ligated into the XbaI site of pSL-UCD, generating pSL-ETG. The plasmid pSL-ETG was cleaved with NdeI and ligated with the 1.8-kb Nde I sacB gene, which was amplified from pCVD442  using the SACB primer pair, producing pSL-ETGS.
In another route, a 1.7-kb apra fragment (apramycin gene), amplified from pSET152  using the APRA primer pair, was cloned into NdeI site of p15A-aph-cat to create p15A-apra-cm. Then, the PCR fragment IR-Tpase-IR or Mx8 was ligated into p15A-apra-cm, which thereafter was cleaved at the 3′ end of the cat gene by ScaI, yielding the plasmid p15A-apra-cm-Tp and p15A-apra-cm-Mx8, respectively. Finally, the 11.5-kb MunI-SpeI fragment from pSL-ETGS with the blunt ends was cloned into the p15A-apra-cm-Tp, which had been cleaved with SnaBI with a blunt end, to create the purposed vector p15A- recTp. The plasmid was cloned into p15A-apra-cm-Mx8, which was further cleaved with KpnI, forming the blunt ends, to create the other purposed vector p15A-recMx8.
The vectors p15A-recTp and p15A-recMx8 was subsequently engineered for recombining with the epothilone gene cluster from Cosmid10 and Fosmid3B11. First, both Cosmid10 and Fosmid3B11 were cleaved with DraI, removing the irrelevant sequences and exposing the terminal sequences with epothilone genes, which contained the identical homologous arms epoup, epoCov and epodown. Then, the linearized Fosmid3B11 was electroporated into the E. coli strain GB05-red harboring p15A-recTp or p15A-recMx8 for the first round of recombination and the transformants with the objective recombinant p15A-TP-fos or p15A-Mx8-fos were selected on the LB plate containing Apra and Cm with extra 10% sucrose for negative selection. Similarly, in the second round the linearized Cosmid10 was electroporated into E. coli GB05-red harboring the plasmid from the first round and the transformants were selected on the LB plate containing extra 1% 2-deoxygalactose (DOG). Ultimately, the engineered recombinant plasmids p15A-Tp-CF and p15A-Mx8-CF containing the integrated epothilone gene cluster were constructed. All the above PCR products were amplified using the pfu DNA polymerase to ensure high fidelity and sequenced to confirm their identities. A diagrammatic sketch for the construction is provided in Figure 2.
Electro-transformation of M. xanthus
The constructed expression plasmids were introduced into M. xanthus cells by electroporation. Briefly, M. xanthus cells from 50-mL overnight cultures (OD600 was about 0.6) were collected and washed thrice with ice-cold water for preparing competent cells. 1.5 mL of the culture was centrifuged and the precipitate was resuspended with 50 μL of ice-cold water in tube, mixed with 3 μg of DNA, and electroporated at a voltage of 1,250 V in a 2 mm cuvette using the Electroporator (Eppendorf, Germany). Then the cells were transferred into 2 mL CYE liquid medium in a 10-mL tube and incubated at 30°C with a rotate speed of 250 rpm. After 4–6 h of incubation, 0.1–0.5 mL of cells was added to 2 mL 0.5% soft agar and the mixture was spread on 1.5% CYE selection agar plates. Resistant colonies that appeared after 6 days of incubation were checked by colony PCR with the following primers: primer pairs of CM-APH and CM-EPOP were used to check the integration of cat gene; Epothilone-specific primers, EA, EP, EB, EC1, EC2, EE and EF, with a 51% coverage of the whole gene cluster, were designed to detect the regions located in ORFs epoA, epoP, epoB, epoC, epoE or epoF, respectively, to verify the integration of the whole biosynthetic gene cluster in the M. xanthus chromosome.
Measurement of chloramphenicol acetyl transferase (CAT) activity in M. xanthus
To measure the chloramphenicol acetyl transferase (CAT) activity, the bacterial pellets were harvested by centrifugation at 8,000×g for 5 min after the cultures reached the mid-exponential phase (36 h of incubation; OD600 was about 3.0). The pellet was resuspended and washed twice with distilled water and then resuspended in 1 mL of lysis buffer. The crude bacterial extracts were prepared by sonication, and the supernatant was collected by centrifugation at 12,000×g for 2 min, and used for characterizing chloramphenicol acetyl transferase (cat) gene expression activity with the CAT ELISA kit, as previously described .
Epothilone extraction and detection
M. xanthus strains containing the complete epothilone gene cluster were grown overnight in 100 mL CYE medium supplemented with Apra and Cm antibiotics. The cultures were inoculated at a ratio of 2:100 into CYE medium containing 2% of the XAD-16 resin for the absorption of epothilone products. After 7 days rotation at the 250 rpm speed and 30°C, the mixtures of cells and resin were harvested by centrifugation and extracted with two volume of methanol by shaking at room temperature overnight . After centrifuged for 20 min, the supernatant was moved into a rotary evaporator to remove the solvent. The residue was further re-dissolved in 1 mL of methanol, and an aliquot of 20 μL of the sample was injected into a Finnigan HPLC system interfaced with a Finnigan MSQ classic quadrupole mass spectrometer (Thermo Finnigan, USA). The analysis was carried out on a Shim-pack MRC-ODS RP column (4.6 mm × 250 mm, 4.60 μm; Shimadzu, Japan) at a temperature of 28°C with a mobile phase of 60% methanol (HPLC grade) and 40% buffer (0.2% acetate acid/18 MΩ Millipore water) at a flow rate of 1.0 mL/min. The MS analysis was performed under the following conditions: ESI-positive, probe temperature of 450°C, cone voltage of 75 V, full scan mass range from 200 to 2,000 m/z at 2 Hz scan speed, and SIM scan at 494 [M + H]+ for epothilone A and 508 [M + H]+ for epothilone B . Epothilones were identified by comparison to the retention time, 249 nm of UV spectra and the MS2 pattern of the authentic reference standard sample containing epothilones A and B, which were obtained from the culture of S. cellulosum So0157-2 as reported previously [31, 33]. The production levels of epothilones were averaged from three independent cultivations and extractions.
Determination of the integration sites
A Genome Walking kit (TaKaRa, Japan) was used to verify the insertion sites of the gene integration. Three specific primers, Cm1, Cm2 and Cm3, designed in this work (Additional file 5: Table S3) were employed with the random primer AP1, which is provided by the kit, in three PCR rounds, to amplify a product containing partial cat region, the IR sequence of the transposon and the flanking unknown genome region of M. xanthus, respectively. Genomic DNA of the transformants with the epothilone gene cluster was extracted and used as the template in the first round PCR using the primer pair AP1/Cm1; the diluted product from the first PCR round was orderly used as the template for the second round, using the primer pair AP1/Cm2; and the product from the second round was diluted as the template for the third PCR round, using the AP1/Cm3 primer pair. The PCR systems and procedures were performed according to the kit protocol. The PCR products were detected by electrophoresis, and the single DNA bands with appropriate size from the third PCR round were withdrawn by the DNA Extraction kit (Promega, USA) and sequenced after subcloning to the pGEM-Teasy vector.
In this study, we exploit the ssRNA-seq method to identify the transcriptional template strands of M. xanthus DZ2 and three transformants ZE-5, ZE-9 and ZE-14 at a whole genome level using the Illumina-platform high throughput sequencing. First of all, the total RNA of the M. xanthus strains were extracted according to the protocol provided by the SV Total RNA isolation system kit (Promega, USA). Residual genomic DNA was removed by treatment with recombinant DNase I (RNase-free; Ambion, USA) according to the manufacturer’s instructions. The quality of the total RNA was verified by agarose gel electrophoresis, and the concentration was determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop technologies, USA). Then, libraries were created by modifying the previously described dUTP second strand method . We fragmented 200 ng of M. xanthus polyA+ RNA by heating at 98°C for 40 min in 0.2 mM sodium citrate, pH 6.4 (Ambion, USA). The fragmented RNA was concentrated to 5 μL, mixed with 3 μg random hexamers, incubated at 70°C for 10 min, and then cooled on ice. The RNA mixtures were further added with 4 μL of 5× first-strand buffer, 2 μL of 100 mM DTT, 1 μL of 10 mM dNTPs, 4 μg of actinomycin D (USB), 200 U SuperScript III, and 20 U SUPERase-In (Ambion, USA), incubated at room temperature for 10 min followed by 1 h at 55°C to synthesize the first-strand cDNA. First-strand cDNA was cleaned up by extraction twice with phenol: chloroform: isoamyl alcohol (25:24:1), followed by ethanol precipitation with 0.1 volumes 5 M ammonia acetate to remove dNTPs and re-suspension in 104 μL ddH2O. Second-strand cDNA was synthesized by adding 4 μL 5× first-strand buffer, 2 μL 100 mM DTT, 4 μL 10 mM dNTPs with dTTP replaced by dUTP (Sigma-Aldrich, USA), 30 μL 5× second strand buffer, 40 U Escherichia coli DNA polymerase, 10 U E. coli DNA ligase, 2 U E. coli RNase H and incubating at 16°C for 2 h. A paired-end library for Illumina sequencing was prepared according to the instructions provided with the following modifications. First, five times less adapter mixture was ligated to the cDNAs. Second, 1 U USER (New England Biolabs, USA) was incubated with 180- to 480-bp size-selected, adapter-ligated cDNA at 37°C for 15 min followed by 5 min at 95°C before PCR. Third, PCR was performed with Phusion High-Fidelity DNA Polymerase with GC buffer (New England Biolabs, USA) and 2 M betaine (Sigma, USA). Fourth, PCR primers were removed using 1.8× volume of AMPure PCR Purification kit (BeckmanCoulter Genomics, USA). Transcriptome sequencing was performed at the BGI Corporation. Reagents were all from Invitrogen (Carlsbad, USA) except as noted.
To assay the growth curves, M. xanthus colonies were first cultured overnight in CYE medium. Then, the cultures were transferred at a ratio of about 2:100 into 5 mL fresh CYE medium with a start OD600 of 0.15, and assayed of the OD600 values periodically.
The reference genome (M. xanthus DK1622) and the gene annotation was retrieved from GenBank (accession no. NC_008095.1). After removing the reads containing sequencing adapters and low-quality reads (reads containing Ns >10%), the remaining 90 bp clean reads with high quality were aligned with the reference genome using Bowtie software . Then the RPKM method was used to normalize the transcript level, which was expressed as the number of reads per kilobase of exon region per million mapped reads (RPKM). Go annotation of the genes was performed using Blast2GO software and visualized by WEGO software.
LPZ, YZL designed the experiments. LPZ, XJY, KH analyzed and interpreted the data. LPZ, XJY, ZFL, LSZ, XNY, HLW performed the experiments. LPZ, YZL wrote the manuscript. YMZ, YZL supervised the research. All authors read and approved the final manuscript.
This work was financially supported by the National Science Foundation of China (NSFC) Key Program [No. 31130004] and National High-tech R&D Program of China (863 Program) [No. 2012AA02A701]. We thank Chun-yan Lin and Guang-rong Zhao from Tianjin University for exchanged communications.
Compliance with ethical guidelines
Competing interests The authors declare that they have no competing interests.
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