Figure 5
From: Human FMO2-based microbial whole-cell catalysts for drug metabolite synthesis
FMO2 based biotransformations of trifluoperazine. a Whole-cell conversions employing E. coli catalysts based on full-length FMO2 and truncations thereof. The conversions were conducted with whole cells corresponding to 15 OD600 units and 1 mM trifluoperazine for 4 h (hatched bars) and 16 h (filled bars), respectively. Values are shown as mean ± SD of measurements performed in triplicates. Cells carrying the empty vector served as negative control (pMS470). b Reaction scheme of the FMO2 catalyzed trifluoperazine oxidation. The corresponding N 1-oxide was identified as product by NMR (see Additional file 1).