Figure 5
From: Genome engineering for improved recombinant protein expression in Escherichia coli
Overall Strategy for host cell engineering using rational and high throughput methods. Leads obtained from analysis of cellular physiology and Omics studies can be used to identify system level bottlenecks. These can be addressed by both single gene modifications or high throughput methods to generate improved platforms for expression. These are initially tested in microbioreactor format to select the best clones for further scale-up.