Figure 4From: Genome engineering for improved recombinant protein expression in Escherichia coli Various pathways for protein translocation (Type I and II). The type II Sec-dependent pathway is the most commonly used and gets overloaded leading to accumation of mis folded proteins in the cytoplasm. However the TAT dependent pathway is used for export of folded proteins and thus requires proper folding in the cytoplasm itself.Back to article page