Figure 5From: Influence of pH control in the formation of inclusion bodies during production of recombinant sphingomyelinase-D in Escherichia coli Kinetics comparison of proteinase-K digestion of rSMD IBs harvested at 3, 5 and 20 h post-induction. A. Digestion of IBs collected under controlled pH. B. Digestion of IBs obtained from uncontrolled pH strategies. The progressive degradation was followed by absorbance and data were normalized. Data show the average of triplicate experiments.Back to article page