Figure 1

Biomass growth and production of sphingomyelinase-D (rSMD) from tick ( Boophilus microplus ) by a recombinant strain of E. coli BL21-Gold (DE3). A. Kinetics of biomass growth for the recombinant strain of E. coli BL21-Gold (DE3). In the inset, the evolution of pH on uncontrolled cultures is shown. Data show the average and the standard deviation of the 24 h cultures that were carried out by quadruplicated. Cultures were carried out under controlled pH at 7.5 (open squares), and uncontrolled pH (closed circles). B. Kinetic behavior of the total protein yield based on biomass dry weight, after chemical induction. Data shows the average and the standard error of duplicate determination of total protein and biomass. C. Kinetic behavior of rSMD yield based on total protein in inclusion bodies, after chemical induction. Data show the average and the standard error from two samples recovered from independent cultures (5, 7 and 9 hours of culture). The average and standard deviation from quadruplicate cultures is shown at 24 h of culture. D. Comparative SDS-PAGE (left) and Western blot (right) of cytoplasmic soluble proteins and those obtained from solubilized IB (with 10% of SDS), obtained at 20 h post-induction (24 h of culture). Lanes 1 and 2, soluble and IB proteins harvested from uncontrolled pH cultures. Lanes 3 and 4, soluble and IB proteins from controlled pH cultures. M means molecular weight marker standard.