Figure 4From: Cloning of the Zygosaccharomyces bailii GAS 1 homologue and effect of cell wall engineering on protein secretory phenotypeTotal proteins released in the culture medium. Total proteins released in the culture medium of batch cultures of the indicated wild type and Δgas 1 strains of S. cerevisiae and Z. bailii. Yeasts were grown in shake flasks in minimal YNB-based medium with 5% glucose. Cells were inoculated at 0.1 OD and harvested after 72 hours of cultivation. Amount of released proteins (in mg l-1 of culture broth) were normalized for OD values.Back to article page