Table 1 Recombinant E. coli strains used in this work. AT or AT144 was used for fumI in the vector names.

E. coli BL21(DE3)

pET-3a-AT

-

E. coli BL21(DE3) pET-3a-AT

pET-3a-HISAT

N-terminal 6xHis tag

E. coli BL21(DE3) pET-3a-HISAT

pET-30a-AT144

-

E. coli BL21(DE3) pET-30a-AT144

pMAL-AT144

MBP

E. coli BL21(DE3) pMAL-AT144

E. coli HMS174(DE3)

pET-3a-HISAT

N-terminal 6xHis tag

E. coli HMS174(DE3) pET-3a-HISAT

pMAL-AT144

MBP

E. coli HMS174(DE3) pMAL-AT144

E. coli Origami 2(DE3)

pET-3a-HISAT

N-terminal 6xHis tag

E. coli Origami 2(DE3) pET-3a-HISAT

pMAL-AT144

MBP

E. coli Origami 2(DE3) pMAL-AT144

pMAL

E. coli Origami 2(DE3) pMAL

E. coli Rosetta 2(DE3)

pET-3a-HISAT

N-terminal 6xHis tag

E. coli Rosetta 2(DE3) pET-3a-HISAT

E. coli Rosetta-gami(DE3)

pET-3a-HISAT

N-terminal 6xHis tag

E. coli Rosetta-gami(DE3) pET-3a-HISAT

E. coli Tuner(DE3)

pET-3a-HISAT

N-terminal 6xHis tag

E. coli Tuner(DE3) pET-3a-HISAT

E. coli ArcticExpress(DE3)

pET-30a-AT144HIS

C-terminal 6xHis tag

E. coli ArcticExpress(DE3) pET-30a-AT144HIS

pET-3a-HISAT

N-terminal 6xHis tag

E. coli ArcticExpress(DE3) pET-3a-HISAT

pET-3a-AT

-

E. coli ArcticExpress(DE3) pET-3a-AT