Figure 4
From: Production of biohydrogen by recombinant expression of [NiFe]-hydrogenase 1 in Escherichia coli
Western blot analysis of recombinant E. coli [NiFe]-hydrogenase 1. Samples were obtained from different cultures according to their metal compositions, as presented. MW: molecular weight standard marker, T: Total cell lysate fraction, IS: insoluble cell debris fraction, S: soluble crude extract fraction, M: membrane fraction. L with arrow indicates the large subunit of [NiFe]-hydrogenase 1, and S represents the small subunit. 25× indicates 25-fold concentrated membrane fraction, based on the total cell lysate concentration. The protein band was detected with an anti-His6 antibody.