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Figure 2 | Microbial Cell Factories

Figure 2

From: Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239

Figure 2

Zymographic and SDS-PAGE analyses. (A) Zymogram. Lane 1, molecular weight markers (Invitrogen Mark12). Lane 2, medium from cultivation of Janthinobacterium sp. 4239. Lane 3, periplasmic extract of E. coli BMS172 diluted 5,000 times. (B) Ordinary SDS polyacrylamide gel. Lane 1, periplasmic extract of reference E. coli strain MGJ1042 diluted 5 times. Lane 2, periplasmic extract of reference E. coli BMS172 diluted 5 times. Lane 3, same periplasmic extract as in lane 3 diluted 50 times. Notice the difference in migration positions of the E. coli BMS172 chitosanase in the two gel systems A and B.

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Microbial Cell Factories

ISSN: 1475-2859

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