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Table 4 Riboflavin accumulation of P. pastoris strains, with an increasing number of deregulated RIB genes

From: Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Strain

Riboflavin in supernatant

X-33 PRC RIB1

6.9 mg/L +/- 0.17

X-33 PRC RIB1+3

9.1 mg/L +/- 0.25

X-33 PRC RIB1+3+7

10.2 mg/L +/- 0.17

X-33 PRC RIB1+3+7+5

13.2 mg/L +/- 0.47

X-33 PRC RIB1+3+7+5+2

14.9 mg/L +/- 0.76

X-33 PRC RIB1+3+7+5+2+4

20.2 mg/L +/- 0.83

  1. The cells were cultivated in shake flasks on defined medium and glucose as carbon source. Shake flask experiments and HPLC measurements were run in duplicates. Mean values +/- standard deviations are given.
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Microbial Cell Factories

ISSN: 1475-2859

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