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Table 3 Riboflavin accumulation of P. pastoris strains, overexpressing two RIB genes

From: Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Strain Riboflavin in supernatant
X-33 ScRIB1 3.9 mg/L +/- 0.25
GS115 PpRIB1 3.0 mg/L +/- 0.10
GS115 PpRIB1+empty 3.0 mg/L +/- 0.18
GS115 PpRIB1+1 7.1 mg/L +/- 0.04
GS115 PpRIB1+3 10.1 mg/L +/- 0.24
GS115 PpRIB1+7 4.9 mg/L +/- 0.19
  1. The cells were cultivated in shake flasks on defined medium and glucose as carbon source. Shake flask experiments and HPLC measurements were run in duplicates. Mean values +/- standard deviations are given.