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Table 3 Riboflavin accumulation of P. pastoris strains, overexpressing two RIB genes

From: Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Strain

Riboflavin in supernatant

X-33 ScRIB1

3.9 mg/L +/- 0.25

GS115 PpRIB1

3.0 mg/L +/- 0.10

GS115 PpRIB1+empty

3.0 mg/L +/- 0.18

GS115 PpRIB1+1

7.1 mg/L +/- 0.04

GS115 PpRIB1+3

10.1 mg/L +/- 0.24

GS115 PpRIB1+7

4.9 mg/L +/- 0.19

  1. The cells were cultivated in shake flasks on defined medium and glucose as carbon source. Shake flask experiments and HPLC measurements were run in duplicates. Mean values +/- standard deviations are given.