Volume 5 Supplement 1

The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

Open Access

Effects of overexpression of X-box binding protein 1 on recombinant protein production in mammalian cells

  • Sebastian Ku1, 3,
  • Grace Chong1,
  • Maybelline Giam1,
  • Miranda GS Yap1, 2, 3 and
  • Sheng-Hao Chao1
Microbial Cell Factories20065(Suppl 1):P90

https://doi.org/10.1186/1475-2859-5-S1-P90

Published: 10 October 2006

Background

X-box binding protein 1 (XBP-1), a key regulator for the cellular secretory pathway, is essential for the differentiation of plasma cells and the unfolded protein response. In the XBP-1 knock-out B primary cells, a profound depression in synthesis and secretion of immunoglobulin M was observed, clearly demonstrating the importance of XBP-1 in protein secretion. There are two protein isoforms of XBP-1, XBP-1S and XBP-1U. The spliced form of XBP-1, XBP-1S, functions as a transcription activator and upregulates many genes associated with protein secretion and biosynthesis of endoplasmic reticula (ER), whereas the unspliced XBP-1U is transcriptionally inactive. Since the production of some recombinant proteins is widely believed to be limited by the secretory capacity of the host cell, we reason that an increase in protein productivity may be achieved by overexpressing XBP-1S in cells. However, XBP-1S is only synthesized when UPR is initiated. To constitutively express XBP-1S in cells, but not XBP-1U, we generated a specific expression plasmid which contains the spliced XBP-1S cDNA. Effects of overexpression of XBP-1S on the productivity of human erythropoietin (hEPO) in CHO-K1 cells were examined.

Results

We hypothesized that protein secretion may become a determinative factor when the production of recombinant proteins exceeds the secretory capacity of host cells. To simulate the saturated condition, CHO-K1 cells were transiently transfected with a hEPO expression vector. 2- to 3-fold increase in hEPO titre was observed when XBP-1S was ectopically expressed in the hEPO-saturated cells. Our findings suggest that the putative saturation of secretory capacity can be alleviated and protein production can be further enhanced by overexpression of XBP-1S.

Conclusion

XBP-1S could be an ideal gene target to improve productivity of recombinant proteins by modulating cellular secretory pathways.

Authors’ Affiliations

(1)
Bioprocessing Technology Institute, Biomedical Sciences Institutes
(2)
Department of Chemical & Biomolecular Engineering, National University of Singapore
(3)
NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore

Copyright

© Ku et al; licensee BioMed Central Ltd. 2006

This article is published under license to BioMed Central Ltd.

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