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Figure 1 | Microbial Cell Factories

Figure 1

From: Increasing the ease and speed of eukaryotic protein expression: a new cell-free in vitro translation system based on Sf insect cell extracts

Figure 1

6xHis-tagged EPO (erythropoietin) and ORM1 (Alpha-1-acid glycoprotein 1) were expressed as 14C-Leu-labeled proteins using the EasyXpress Protein Synthesis Insect Kit. After expression an aliquot of each was treated with the endoglycosidase EndoH, which removes glycan moieties form glycosylated proteins. Aliquots of treated and untreated protein separated by SDS_PAGE and proteins visualized by autoradiography.

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Microbial Cell Factories

ISSN: 1475-2859

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