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Figure 5 | Microbial Cell Factories

Figure 5

From: The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies

Figure 5

Disaggregation and resolubilisation of inclusion bodies produced at 42°C Inclusion bodies of α-glucosidase were produced for two hours at 42°C, followed by addition of tetracycline and subsequent incubation at 30°C. Coomassie-stained SDS-PAGE gel of (A) the insoluble cell fractions and (B) the soluble cell fractions extracted from identical amounts of biomass; times relative to induction and ibpAB genotype are given above the lanes. Positions of α-glucosidase, IbpA/IbpB and membrane proteins are indicated. First lane contains molecular weight markers of 97.4, 66.2, 45, 31, 21.5 and 14.4 kDa, respectively.

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Microbial Cell Factories

ISSN: 1475-2859

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