Skip to main content

Lactobacilli-Host mutualism: "learning on the fly"


Metazoans establish with microorganisms complex interactions for their mutual benefits. Drosophila, which has already proven useful host model to study several aspects of innate immunity and host-bacteria pathogenic associations has become a powerful model to dissect the mechanisms behind mutualistic host-microbe interactions. Drosophila microbiota is composed of simple and aerotolerant bacterial communities mostly composed of Lactobacillaceae and Acetobactereaceae. Drosophila mono- or poly-associated with lactobacilli strains constitutes a powerful model to dissect the complex interplay between lactobacilli and host biologic traits. Thanks to the genetic tractability of both Drosophila and lactobacilli this association model offers a great opportunity to reveal the underlying molecular mechanisms. Here, we review our current knowledge about how the Drosophila model is helping our understanding of how lactobacilli shapes host biology.


Metazoans establish with microorganisms complex interactions for their mutual benefits. When the host-bacteria system is in balance or homeostasis, it can contribute for many aspects of host physiology [1]. As an example, the gut bacterial communities or microbiota improve the digestion of nutrients and provide new metabolic functions to their host. They also contribute to the education and stimulation of the immune system and are responsible for colonization resistance against pathogens [2]. On the bacterial side, host is furnishing a niche with accessibility to substrates either exogenous from food or endogenous from mucus. However, when the tight balance between host and microbiota is broken it can result in the development of certain pathologies [1].

Over the last 15 years, the composition of the gut microbiota has been explored by culture-independent techniques [3] and further described by metagenomic analysis [4, 5]. Analyses of the gene encoding 16S rRNA by pyrosequencing have identified up to ten phyla represented in microbiota. At a lower taxonomic level, up to a thousand species are generally present in one individual. Metagenomic analysis has revealed, within the inter-individual variation, the presence of less than sixty species shared among human individuals [5]. The relative proportion of each species is influenced by environmental and host factors [6]. Despite the ongoing interest on the gut microbiota, the mechanisms behind the interactions between host and microbes remain to be better understood. This comprehension is hampered by the complexity and variability of the bacterial communities involved in mammalian host-microbe interactions. In addition, most of the bacteria of the human gastrointestinal tract have not yet been cultured ex-vivo, due in part to their anaerobic metabolism or sensitivity to oxygen; those that have been cultured require laborious techniques [7]. Thus, the use of simpler animal models may help to unravel evolutionary conserved mechanisms underlying the impact of intestinal bacterial in their host physiology. In this light, Drosophila, which has already proven useful in the study of several aspects of innate immunity and host-bacteria pathogenic associations [8], has become a powerful model to dissect the mechanisms behind mutualistic host-microbe interactions (Figure 1) [9, 10]. Drosophila combines genetic and experimental tractability with a culturable microbiota of low diversity that facilitates microbial genetic analysis.

Figure 1
figure 1

Functional impact of commensal lactobacilli on Drosophila biology. Strains of L. plantarum can be true mutualists and impact different aspects of host biology: mating preferences, gut epithelial homeostasis, protection against enteric infection, nutrient allocation and metabolism. During larval stages it promotes systemic growth. Depending on the strain and host genetic background, L. brevis is a mutualist of pathobiont, which interferes with host metabolism and/or induces chronic inflammation and epithelial cell death.

Drosophila gut microbiota composition: high prevalence of lactobacilli

Several studies have determined the composition of Drosophila gut commensal bacterial communities and the factors that shape their diversity and relative abundance. Overall Drosophila microbiota is composed of simple bacterial communities represented by the phyla Firmicutes (Lactobacillaceae and Enterococcaceae) and Proteobacteria (Acetobactereaceae and Enterobactereaceae) with five dominating species: Acetobacter pomorum, A. tropicalis, Lactobacillus brevis, L. plantarum and L. fructivorans [11, 12]. With the exception of Acetobactereaceae, these species are also commensals in humans [5]. Strains of Gluconobacter morbifer and more recently L. brevis were identified as Drosophila pathogens that under certain circumstances can act as a colitogenic pathobionts due to their ability to constantly release uracil [11, 13]. Uracil release by pathogenic bacteria and certain opportunistic pathobionts was identified as the ligand inducing DUOX-dependent reactive oxygen species generation in Drosophila's gut, which is required for the efficient elimination of bacteria but also causes inflammation and cell death. In the case of these opportunistic pathobionts, the permanent release of uracil and subsequent DUOX activation leads to chronic inflammation [13].

Studies to determine Drosophila gut microbiota composition were carried out in whole flies or dissected guts from laboratory-raised and wild-caught flies. The diversity was determined using both culturable-dependent and culture-independent techniques such as amplification and sequencing of microbial 16S rRNA. Globally they report that Drosophila gut bacterial communities are sensitive to variations on diet, developmental stage and host immune status [11, 12, 14, 15].

Clearly, the most important factor shaping Drosophila's gut microbiota is the diet. Corby-Harris investigated community richness and composition of 11 fly populations recovered from different latitudes. Although the authors observed significant spatial variation in microbial community richness they could not find a clear relationship between latitude or climate and microbial richness [16]. Likewise, the analysis of natural bacterial communities associated with different species of Drosophila collected from distant geographical locations revealed that the gut microbiota composition of different species feeding on the same type of substrates were more similar to each other then to more closely related species feeding on different substrates [14, 17]. In those studies the differences in microbial content were attributed to food composition. Supporting these observations, it was shown that increasing the carbohydrate to protein ratio in the medium enhances the proportion of Acetobacter versus Lactobacillus in young adult flies [12, 18] and that changes on diet such as high-fat or short- term starvation have drastic and long-lasting effects on the microbiota [19]. Although the major represented groups detected in laboratory-raised flies were found to be present in most of wild Drosophila populations, lab-reared flies and wild-caught flies diverge in their microbiota composition. Moreover, bacterial communities associated with lab-reared flies are striking less diverse than those of wild-caught flies. Thus, so far, it was not possible to define a group of microorganisms consistently recovered from Drosophila that we could call a core microbiota [14, 15, 17]. Nevertheless it is reasonable to think that taxonomically variable bacterial communities can be functionally equivalent and instead of a core microbiota, Drosophila would sustain a core microbiome.

Other factors shaping Drosophila's gut microbiota composition are developmental status and aging. Indeed, a case study from the Douglas' lab indicated that despite bacterial species presence all along Drosophila's life cycle, their abundance varies with developmental age. During larval growth the dominant species in the fly line they studied changes from L. fructivorans to L. plantarum. In the same line, at pupal stages A. tropicalis was the most represented species while in young adults, L. fructivorans became dominant again and in old adults A. pomorum was the dominant species in the community [12]. It has been suggested that the gut oxidative status could be driving species predominance during Drosophila's life cycle favouring either aerobic or aerotolerant bacteria growth or it may reflect different nutritional needs and immune performances [9, 12]. In fact, old flies have their bacterial loads increased, which has been associated to age-related decrease in the efficiency of immune responses [20, 21]. However the global composition and abundance of the gut microbiota in Drosophila is extremely variable among different laboratories and among Drosophila adults within the same line [17], so this issue yet deserves further investigation. Finally, host genetic determinants can also impact the density and composition of gut bacterial communities, as has been demonstrated by Ryu and colleagues [11]: flies with higher antimicrobial peptides production due to a mutation on the homeobox gene Caudal (Cad) show a shift in community composition from the commensal bacteria Commensalibacter intestini to a minor member of Drosophila's gut, Gluconobacter morbifer, recognized as a pathobiont.

Despite the numerous studies on Drosophila's gut microbiota composition and the general agreement of the community in that the most represented species are member of the genus Lactobacillus and Acetobacter, their global composition and abundance remains extremely variable among wild-caught and lab-raised flies as well as between and within different laboratories over time [17]. Moreover, a recent study by Blum and colleagues determine how Drosophila's gut microbiota is established and maintained. They evaluate bacterial communities of newly emerged flies transferred to fresh food daily for 3 days or kept in the same food for 7 days and realise that flies that were not transferred harboured larger bacterial populations than those that were transferred. In this way they confirmed that Drosophila need to consume bacteria from the environment in order to establish and maintain its microbiota, which indicate that bacteria do not persist long in Drosophila's gut [22]. These two aspects should be taken into account when working with Drosophila since they can have an impact on observed phenotypes.

As mentioned before, lactobacilli are prevalent commensals of Drosophila melanogaster similarly to mammals including humans. In Drosophila, they reside in the intestine and are vertically transmitted to progenies via the deposition of contaminated mother's faeces on the surface of the embryo during egg laying and on the surrounding substratum. Many strains of different lactobacilli species (L. plantarum, L. brevis, L. fructivorans) can colonize germ-free animals and remained associated to their host during its entire life cycle by constant re- association through ingestion. In addition to being commensal species of Drosophila melanogaster intestine, lactobacilli have been used for decades as a model lactic acid bacteria and therefore offers vast technical resources and potential [23]. Therefore the approach of using lactobacilli and an animal host model such as Drosophila with evolutionary conserved genetic and physiological features to study intestinal host-microbe interactions is well suited to unravel lactobacilli/host interactions encountered in the wild potentially including those occurring in humans.

Functional impact of lactobacilli on Drosophila biology

Impact on host behaviour

Sharon and colleagues investigated if diet could impact fly's behaviour such as mating preferences. Flies reared in cornmeal-molasse-yeast medium (CMY, a high carbohydrate to protein ratio diet containing simple sugars) were split in two groups: one group was kept in CMY medium whereas the other was shifted to starch medium (lower carbohydrate/protein ratio without addition of simple sugars). After few generations they were transferred to mating chambers and tested for mating preferences [24]. Flies are more sexually attracted to individuals fed on the same diet showing assortative mating (non-random). This preference seems to be dependent on the gut microbiota composition. Indeed, starch diet-fed flies harbour 10 times more lactobacilli than CMY-fed flies (confirming the strong impact of diets on microbiota composition). This sexual preference was abolished by antibiotic treatment since flies behaviour passed from positive assortative to random after treatment. The analysis of the bacterial communities associated with flies reared in each medium revealed a strong association of flies reared on starch diet with L. plantarum. Subsequent experiments revealed a preference of ex-germ-free, L. plantarum monoassociated flies to mate with starch-diet fed flies (carrying high prevalence of L. plantarum) in detriment of CMY-fed flies [24]. The analysis of the cuticular hydrocarbon (communication clues for insects) composition of axenic flies and conventional flies reared in CMY and starch medium showed significant differences among flies harbouring different gut microbiota. All together the results from this work suggest that symbiotic bacteria, and in particular L. plantarum can influence mating preferences by changing the levels of cuticular hydrocarbon sex pheromones establishing a link between environment (diet), microbiome and behaviour. However, the exact mechanism by which bacteria influence mating preferences remains to be elucidated [24]. Similarly, a recent paper by the Dukas' lab showed that Drosophila larvae associated to lactobacilli (L. brevis and L .plantarum strains) significantly attracted adult flies or other larvae to their hospitable nutritional niche but were non attractive to others if they lacked a microbiota [24, 25]. These observations suggest again that the host microbiota mediates behavioural responses, yet the underlying mechanism remains elusive but the Drosophila model which has historically been the premier model system for understanding the molecular and genetic bases of complex behaviours [26] offers great promise to identify them.

Protection against infection

A major function of the gut microbiota is the protection against colonization by pathogens and the control of pathobionts overgrowth. It was already observed that certain members of the fly gut microbiota provide protection against pathobiont overgrowth [11]. Recently, Blum and colleagues proposed that the gut microbiota can also protect the fly against infection by a pathogen [22]. Conventional (CONV) and germ-free flies associated with a strain of L. plantarum were less susceptible than germ-free animals to gut infection by Serratia marcescens. The authors suggest that the protection is correlated with L. plantarum density in the flies that is higher in associated ex-germ-free flies than associated-CONV flies. In order to enlarge the number of species tested, the authors evaluated the level of protection given by Enterococcus faecalis and conclude that it does not protect flies against infection by these pathogenic bacteria. Although only one strain of L. plantarum and E. faecalis were tested, the authors claim that the protective effect seems L. plantarum specific. Further studies with higher number of strains should be conducted to better elucidate the specificity of the phenotype as well as determine how L. plantarum exerts its protective effect [22]. Given the interest of using lactobacilli as human probiotics, the authors evaluated the protective effect of Lactobacillus rhamnosus (which is not a Drosophila commensal) against S. marcescens and Pseudomonas aeruginosa. As S. marcescens is a fly and human pathogen and strains of L. plantarum and L. rhamnosus are used as probiotics in humans and had a protective effect against infections in Drosophila, the authors suggest that the Drosophila-lactobacilli association model might be useful to unravel probiotic effects of different lactobacilli strains since they may be conserved and further translated to humans [22]. This seems a valuable model, however more complete and detailed studies need to be performed in support of these exciting preliminary observations.

Gut epithelial homeostasis

In order to maintain homeostasis, the gut epithelium is continuously replenished by stem cells. The disruption of this equilibrium may result in disease [27]. In Drosophila, midgut cells are replenished by a population of intestinal stem cells (ISCs) which division generates a new ISC and a post-mitotic enteroblast that differentiates into an adsorptive enterocyte or a secretory enteroendocrine cell [28]. Buchon and colleagues established for the first time that the gut microbiota stimulates a basal level of stem cell activation and subsequent epithelium renewal since axenic animals present slower intestinal epithelial cell renewal rate than conventional flies [21]. The similarities between Drosophila and mammals ISCs can promote the genetic analysis of normal and abnormal intestinal function in a simpler model and can help to elucidate the role of the microbiota in gut homeostasis [28].

The work by Jones and colleagues further characterize how the gut microbiota affects epithelial renewal and identify that lactobacilli is particularly prone to do so [29]. The authors first demonstrated that the association of germ-free Drosophila with L. plantarum induces ROS generation by midgut enterocytes. Association with other members of the gut microbiota did not elicit the same response. Since L. plantarum, a commensal bacteria of the Drosophila's gut, is able to induce ROS production in the intestine, the authors investigate the involvement of dNOX and dDUOX for this phenotype, the only two NADPH oxidases in Drosophila. Through the midgut specific expression of dsRNA targetting dNOX and dDUOX the authors identified that L. plantarum induced ROS production is dependent on dNOX.

Finally they determined that L. plantarum induce ROS-dependent cellular proliferation in the Drosophila intestine [29]. It is interesting to note that a previous work by Lee and colleagues detected DUOX-dependent ROS production induced by pathogenic bacteria and pathobionts in Drosophila's gut [13]. It is hard to integrate both results since the time of detection and the compounds used to detect ROS production were not the same. In fact, Lee and colleagues detected a stronger form of ROS, HOCl, and Jones and colleagues used a compound that detects a broader range of ROS [13, 29]. Further studies should conciliate these two sets of data.

In sum, the authors demonstrated that lactobacilli are strong inducers of endogenous ROS generation and ROS-dependent cellular proliferation within Drosophila intestines but also mammals. These two effects are dependent on a functional Nox enzyme in intestinal epithelial cells. It was demonstrated with this work that lactobacilli-induced ROS generation is conserved in metazoans, which strengths the use of Drosophila simple model to study intestinal homeostasis since the mechanisms are probably conserved in mammals [29]. Future research should target the molecular mechanisms behind lactobacilli promotion of epithelial homeostasis in both bacteria and host.

Impact on nutrition and related metabolism

The nutritional status of animals is deeply influenced by the gut microbiota that can provide supplementary nutrients to the host, alter nutrient assimilation and allocation patterns [30]. Several recent studies addressed the question of how Drosophila gut microbiota shapes host nutritional traits [18, 31, 32].

Ridley and colleagues compared host performance, nutritional status and metabolic rate of conventional flies harboring a microbiota dominated by A. pomorum and axenic flies. This work revealed that axenic flies have higher glucose, trehalose and glycogen contents than conventional flies demonstrating that the gut microbiota impacts the carbohydrate allocation patterns of adult Drosophila [18]. Very recently Wong and colleagues investigated the combine effect of diet and absence of the intestinal microbiota on Drosophila performance and nutrient allocation [32]. The authors showed that the intestinal microbiota supports Drosophila performance on diets of low or unbalanced nutrient content but also that it is implicated in vitamin B and protein nutrition as well as energy storage [32].

To understand the contribution of individual microbial strains and interactions between strains in shaping nutrient allocation, Newell and Douglas tested strains of the 5 most common species in Drosophila's intestinal microbiota (A. pomorum, A. tropicalis, L. brevis, L. plantarum and L. fructivorans) in monoassociation or poly-association with Drosophila [31]. The authors compared conventional flies, 5-species microbiota associated flies and flies monoassociated with each one of the 5 strains in several nutritional parameters: adult weight and protein, glucose, glycogen and TAG contents. All 5 species reduced circulating glucose levels to that of conventional flies. Concerning TAG content, none of the conditions with lactobacilli recapitulated conventional flies content, nevertheless L. plantarum treatment reduced slightly TAG content when compared with axenic flies. Flies monoassociated with Acetobacter species showed reduced TAG levels but only the 5-species microbiota recapitulate conventional flies TAG content, which suggests interaction among species for this host phenotype. Those results led the authors to test the impact of all possible pairwise Acetobacter-Lactobacillus combinations on nutritional parameters. Interestingly, the pair A. tropicalis-L. brevis treatment reduced TAG to levels significantly lower than 5-species microbiota revealing a very strong effect of this bacterial association. Likewise, this pair was also the most efficient in lowering flies weight. The authors suggest that Acetobacter- mediated reduction of host TAG is promoted by co-colonization with Lactobacillus species, which revels a phenotype that is dependent on interspecies interaction inside Drosophila's intestine that needs to be further studied [31].

The studies mentioned above confirmed the impact of Drosophila gut microbiota on several aspects of host nutrition, including nutrient allocation patterns and highlights the importance of gut microbiota composition in host metabolic phenotypes.

Impact on host development

Several studies have addressed the impact of Drosophila intestinal microbiota for systemic growth and unravel some of the mechanism underlying this phenotype [18, 31, 33, 34]. Globally, those studies revealed that on a conventional diet rich in simple sugars and proteins, axenic flies emerge approximately 2 days later than conventional flies. However, under nutrient scarcity such developmental delay is massively increased [18, 31, 33, 34]. Clearly, Drosophila systemic growth is influenced by nutrient availability as well as intestinal microbiota composition. Thus the presence of gut microbiota should be particularly useful under harsh condition that flies might face in the wild. In order to narrow down the effects of the microbiota on Drosophila systemic growth to a specific member of the consortium or interactions amongst individual species, Newell and Douglas evaluated the contribution of strains of the 5 most common species in Drosophila's intestinal microbiota (A. pomorum, A. tropicalis, L. brevis, L. plantarum and L. fructivorans) to host developmental time [31]. They evaluated the effect of the defined 5-strains microbiota (poly-association) and the effect of each strain individually upon mono-association with Drosophila. Both mono-association of A. pomorum and A. tropicalis are able to produce developmental times comparable to those of the 5-strains gut microbiota whereas L. plantarum and L. brevis have an intermediate effect and L. fructivorans has no visible effect [31]. It should be noted that as the diet influences gut microbiota composition and activity, which fluctuates depending on carbohydrates to protein ratio and levels of simple sugars, the higher level of simple sugars in the diet used in Newell's paper might have hampered the prevalence and activity of lactobacilli and therefore their impact on Drosophila's growth. Indeed, Acetobacter predominates on simple sugar rich diet compare to lactobacilli [33, 34]. Two other studies revealed the contribution of Drosophila microbiota to its host systemic growth [33, 34]. Shin and colleagues, using a simple sugar containing diet, revealed the marked impact of strains of Acetobactereaceae species (Acetobacter pomorum, Commensalibacter intestini and Gluconobacter morbifer). They also identified the growth promoting effect, albeit less marked than Acetobactereaceae, of Lactobacillus plantarum and L. brevis strains. Then, Shin et al. mainly worked on a monoassociation Acetobacter-Drosophila model and evidenced both host and bacterial factors underlying growth promotion effect but also energy metabolism and intestinal stem cell activity [33]. In fact, production of acid acetic by the bacterial pyrroloquinonoline quinone- dependent alcohol dehydrogenase (PQQ-ADH) modulates insulin/insulin-like growth factor signalling (IIS) in Drosophila leading to the control of host nutritional signalling networks. However more bacterial functions might be involved since the simple addition of acetic acid to the medium did not restore the growth promotion effect [33]. In the case of Storelli and colleagues, standard fly diets containing inactivated yeast and starch from cornmeal and devoid of simple sugar addition were used and L. plantarum, the most prevalent bacterial species in these flies (no Acetobactereaceae were detected) and was able to promote larval growth upon nutrient scarcity as efficiently as a control microbiota resulting in early adult emergence [34]. L. plantarum beneficial effect is strain specific since other strains were not able to promote larval growth in similar conditions pointing to the existence of specific bacterial activities present in growth promoting strains that non-growth promoting strains lack which are essential for the Drosophila growth phenotype to express [34]. L. plantarum exerts its beneficial effect on larval growth through the host nutrient sensing system that relies on the tissue specific activity of the TOR kinase that subsequently modulates hormonal signals controlling growth and maturation. Stimulation of TOR kinase activity by diet-derived branched-chain amino acids in the fat body leads to increased Drosophila insulin-like peptides (dILPs) production by the brain [35]. At the same time, activation TOR kinase activity in the prothoracic gland, promotes Ecdysone production during late larval stage and impacts on the length of the growth phase [34]. Efforts in our lab are on-going to decipher how L. plantarum promotes TOR activity; L. plantarum-mediated enhanced digestion and absorption of peptides from the diet leading to optimization of diet-derived branched-chain amino acids in the hemolymph is the current working hypothesis.


Drosophila melanogaster has emerged as a powerful model to study host-gut microbiota mutualism given the simplicity and low-complexity of its microbial communities and the ease to generate and maintain gnotobiotic animals. Drosophila mono- or poly-associated with lactobacilli strains under certain nutritional conditions constitutes a powerful model to dissect the complex interplay between diet, bacteria and host biologic traits and thanks to the genetic tractability of both Drosophila and lactobacilli this model offers a great opportunity to reveal the underlying molecular mechanisms. Given its contribution to unravel TOR and IIS pathways involvement in Lactobacillus plantarum mediated growth promotion, this model will also help dissecting if and how other lactobacilli strains, including available probiotic strains promote juvenile growth and/or influence host metabolism paving the way to the use, the identification or the design of next generation evidence-based probiotic strains. In addition, given its pioneer role in the study of the fundamental roots of innate immunity and host/pathogen interactions [8], the Drosophila model may also offer opportunities to study the interplay between lactobacilli, metabolism and the innate immune system. Finally, studies on the Drosophila microbiota/gut/brain axis [36] and its impact on host behaviour [24, 25] offer exciting and refreshing perspectives to the lactobacilli/Host interaction field.



ribosomal ribonucleic acid




intestinal stem cell


reactive oxygen species




insulin/insulin-like growth factor signalling


pyrroloquinonoline quinone-dependent alcohol dehydrogenase


target of rapamycin


insulin-like peptides.


  1. Clemente JC, Ursell LK, Parfrey LW, Knight R: The Impact of the Gut Microbiota on Human Health: An Integrative View. CELL. 2012, 148: 1258-1270.

    Article  CAS  Google Scholar 

  2. Fraune S, Bosch TCG: Why bacteria matter in animal development and evolution. Bioessays. 2010, 32: 571-580.

    Article  CAS  Google Scholar 

  3. Lozupone CA, Stombaugh JI, Gordon JI, Jansson JK, Knight R: Diversity, stability and resilience of the human gut microbiota. Nature. 2012, 489: 220-230.

    Article  CAS  Google Scholar 

  4. Arumugam M, Raes J, Pelletier E, Le Paslier D, Yamada T, Mende DR, Fernandes GR, Tap J, Bruls T, Batto J-M, Bertalan M, Borruel N, Casellas F, Fernandez L, Gautier L, Hansen T, Hattori M, Hayashi T, Kleerebezem M, Kurokawa K, Leclerc M, Levenez F, Manichanh C, Nielsen HB, Nielsen T, Pons N, Poulain J, Qin J, Sicheritz-Ponten T, Tims S, et al.: Enterotypes of the human gut microbiome. Nature. 2011, 473: 174-180.

    Article  CAS  Google Scholar 

  5. Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, Nielsen T, Pons N, Levenez F, Yamada T, Mende DR, Li J, Xu J, Li S, Li D, Cao J, Wang B, Liang H, Zheng H, Xie Y, Tap J, Lepage P, Bertalan M, Batto J-M, Hansen T, Le Paslier D, Linneberg A, Nielsen HB, Pelletier E, Renault P, et al.: A human gut microbial gene catalogue established by metagenomic sequencing. Nature. 2010, 464: 59-65.

    Article  CAS  Google Scholar 

  6. Spor A, Koren O, Ley R: Unravelling the effects of the environment and host genotype on the gut microbiome. Nat Rev Microbiol. 2011, 9: 279-290.

    Article  CAS  Google Scholar 

  7. Duncan SH, Louis P, Flint HJ: Cultivable bacterial diversity from the human colon. Lett Appl Microbiol. 2007, 44: 343-350.

    Article  CAS  Google Scholar 

  8. Lemaitre B, Hoffmann J: The Host Defense of Drosophila melanogaster. Annu Rev Immunol. 2007, 25: 697-743.

    Article  CAS  Google Scholar 

  9. Erkosar B, Storelli G, Defaye A, Leulier F: Host-Intestinal Microbiota Mutualism: "Learning on the Fly". Cell Host and Microbe. 2013, 13: 8-14.

    Article  CAS  Google Scholar 

  10. Lee K-A, Lee W-J: Drosophila as a model for intestinal dysbiosis and chronic inflammatory diseases. Developmental and Comparative Immunology. 2014, 42: 102-110.

    Article  CAS  Google Scholar 

  11. Ryu JH, Kim SH, Lee HY, Bai JY, Nam YD, Bae JW, Lee DG, Shin SC, Ha EM, Lee WJ: Innate Immune Homeostasis by the Homeobox Gene Caudal and Commensal-Gut Mutualism in Drosophila. Science. 2008, 319: 777-782.

    Article  CAS  Google Scholar 

  12. Wong CNA, Ng P, Douglas AE: Low-diversity bacterial community in the gut of the fruitfly Drosophila melanogaster. Environmental Microbiology. 2011, 13: 1889-1900.

    Article  CAS  Google Scholar 

  13. Lee K-A, Kim S-H, Kim E-K, Ha E-M, You H, Kim B, Kim M-J, Kwon Y, Ryu J-H, Lee W-J: Bacterial-Derived Uracil as a Modulator of Mucosal Immunity and Gut-Microbe Homeostasis in Drosophila. CELL. 2013, 153: 797-811.

    Article  CAS  Google Scholar 

  14. Chandler JA, Morgan Lang J, Bhatnagar S, Eisen JA, Kopp A: Bacterial Communities of Diverse Drosophila Species: Ecological Context of a Host-Microbe Model System. PLoS Genet. 2011, 7: e1002272-

    Article  CAS  Google Scholar 

  15. Staubach F, Baines JF, Künzel S, Bik EM, Petrov DA: Host Species and Environmental Effects on Bacterial Communities Associated with Drosophila in the Laboratory and in the Natural Environment. PLoS ONE. 2013, 8: e70749-

    Article  CAS  Google Scholar 

  16. Corby-Harris V, Pontaroli AC, Shimkets LJ, Bennetzen JL, Habel KE, Promislow DEL: Geographical Distribution and Diversity of Bacteria Associated with Natural Populations of Drosophila melanogaster. Applied and Environmental Microbiology. 2007, 73: 3470-3479.

    Article  CAS  Google Scholar 

  17. Wong AC-N, Chaston JM, Douglas AE: The inconstant gut microbiota of Drosophila species revealed by 16S rRNA gene analysis. The ISME Journal. 2013, 7: 1922-1932.

    Article  CAS  Google Scholar 

  18. Ridley EV, Wong AC-N, Westmiller S, Douglas AE: Impact of the Resident Microbiota on the Nutritional Phenotype of Drosophila melanogaster. PLoS ONE. 2012, 7: e36765-

    Article  CAS  Google Scholar 

  19. Fink C, Staubach F, Kuenzel S, Baines JF, Roeder T: Noninvasive Analysis of Microbiome Dynamics in the Fruit Fly Drosophila melanogaster. Applied and Environmental Microbiology. 2013, 79: 6984-6988.

    Article  CAS  Google Scholar 

  20. Guo L, Karpac J, Tran SL, Jasper H: PGRP-SC2 Promotes Gut Immune Homeostasis to Limit Commensal Dysbiosis and Extend Lifespan. CELL. 2014, 156: 109-122.

    Article  CAS  Google Scholar 

  21. Buchon N, Broderick NA, Chakrabarti S, Lemaitre B: Invasive and indigenous microbiota impact intestinal stem cell activity through multiple pathways in Drosophila. Genes & Development. 2009, 23: 2333-2344.

    Article  CAS  Google Scholar 

  22. Blum JE, Fischer CN, Miles J, Handelsman J: Frequent Replenishment Sustains the Beneficial Microbiome of Drosophila melanogaster. mBio. 2013, 4: e00860-13-e00860-13.

    Article  Google Scholar 

  23. Kleerebezem M, Boekhorst J, van Kranenburg R, Molenaar D, Kuipers OP, Leer R, Tarchini R, Peters SA, Sandbrink HM, Fiers MWEJ, Stiekema W, Lankhorst RMK, Bron PA, Hoffer SM, Groot MNN, Kerkhoven R, de Vries M, Ursing B, de Vos WM, Siezen RJ: Complete genome sequence of Lactobacillus plantarum WCFS1. Proc Natl Acad Sci USA. 2003, 100: 1990-1995.

    Article  CAS  Google Scholar 

  24. Sharon G, Segal D, Ringo JM, Hefetz A, Zilber-Rosenberg I, Rosenberg E: Commensal bacteria play a role in mating preference of Drosophila melanogaster. Proc Natl Acad Sci USA. 2010, 107: 20051-20056.

    Article  CAS  Google Scholar 

  25. Venu I, Durisko Z, Xu J, Dukas R: Social attraction mediated by fruit flies' microbiome. Journal of Experimental Biology. 2014, 217: 1346-1352.

    Article  Google Scholar 

  26. Griffith LC: Identifying behavioral circuits in Drosophila melanogaster: moving targets in a flying insect. Current Opinion in Neurobiology. 2012, 22: 609-614.

    Article  CAS  Google Scholar 

  27. Pédron T, Sansonetti P: Commensals, Bacterial Pathogens and Intestinal Inflammation: An Intriguing Ménage à Trois. Cell Host and Microbe. 2008, 3: 344-347.

    Article  Google Scholar 

  28. Ohlstein B, Spradling A: The adult Drosophila posterior midgut is maintained by pluripotent stem cells. Nature. 2005, 439: 470-474.

    Article  Google Scholar 

  29. Jones RM, Luo L, Ardita CS, Richardson AN, Kwon YM, Mercante JW, Alam A, Gates CL, Wu H, Swanson PA, Lambeth JD, Denning PW, Neish AS: Symbiotic lactobacilli stimulate gut epithelial proliferation via Nox-mediated generation of reactive oxygen species. The EMBO Journal. 2013, 32: 3017-3028.

    Article  CAS  Google Scholar 

  30. Flint HJ, Scott KP, Louis P, Duncan SH: The role of the gut microbiota in nutrition and health. Nature Reviews Gastroenterology & Hepatology. 2012, 9: 577-589.

    Article  CAS  Google Scholar 

  31. Newell PD, Douglas AE: Among-species interactions determine the impact of gut microbiota on nutrient allocation in Drosophila melanogaster. Applied and Environmental Microbiology. 2013

    Google Scholar 

  32. Wong ACN, Dobson AJ, Douglas AE: Gut microbiota dictates the metabolic response of Drosophila to diet. Journal of Experimental Biology. 2014

    Google Scholar 

  33. Shin SC, Kim SH, You H, Kim B, Kim AC, Lee KA, Yoon JH, Ryu JH, Lee WJ: Drosophila Microbiome Modulates Host Developmental and Metabolic Homeostasis via Insulin Signaling. Science. 2011, 334: 670-674.

    Article  CAS  Google Scholar 

  34. Storelli G, Defaye A, Erkosar B, Hols P, Royet J, Leulier F: Lactobacillus plantarum Promotes Drosophila Systemic Growth by Modulating Hormonal Signals through TOR-Dependent Nutrient Sensing. Cell Metabolism. 2011, 14: 403-414.

    Article  CAS  Google Scholar 

  35. Colombani J, Raisin S, Pantalacci S, Radimerski T, Montagne J, Léopold P: A nutrient sensor mechanism controls Drosophila growth. CELL. 2003, 114: 739-749.

    Article  CAS  Google Scholar 

  36. Lemaitre B, Miguel-Aliaga I: The Digestive Tract of Drosophila melanogaster. Annu Rev Genet. 2012, 47: 130909162859001-

    Google Scholar 

Download references


RCM thanks the "Fondation pour la Recherche Médicale" for financial support through a postdoctoral scholarship SPF20140129318. Research in FL's lab is supported by an ERC starting grant (FP7/2007-2013-N°309704) and is sponsored by the ATIP/AVENIR program, the FINOVI foundation, and the "Fondation Schlumberger pour l'Education et la Recherche soutenue par la FRM".

This article has been published as part of Microbial Cell Factories Volume 13 Supplement 1, 2014: Proceedings of the 11th International Symposium on Lactic Acid Bacteria. The full contents of the supplement are available online at Publication charges for this supplement were funded by the Stichting Symposium on Lactic Acid Bacteria. The articles have undergone the journal's standard peer review process for supplements. EJ is employed by Chr Hansen A/S, is a shareholder in Chr Hansen A/S; and has applied for patents relating to the content of an article which he did not edit but which forms part of this supplement. MK is an employee of Nizo. The other Supplement Editors declare that they have no competing interests.

Author information

Authors and Affiliations


Corresponding author

Correspondence to François Leulier.

Additional information

Competing interests

The authors declare that they have no competing interests.

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Matos, R.C., Leulier, F. Lactobacilli-Host mutualism: "learning on the fly". Microb Cell Fact 13 (Suppl 1), S6 (2014).

Download citation

  • Published:

  • DOI: