Design and implementation of a high yield production system for recombinant expression of peptides

Table 1 Purification of KSI–p53pAnt fused peptide and p53pAnt peptide, overexpressed in E. coli BL21(DE3)

Purification step	Total fused peptide (mg)	Total p53pAnt (mg)	Purity (%)	Yield (%)
Total protein extract	n.d	n.d	43*	100
Inclusion bodies	n.d	n.d	79*	-
First affinity chromatography	184**	43	90*	-
Precipitation recovery	184	43	-	100*
Thrombin cleavage	-	-	-	95*
Second affinity chromatography	-	34**	95	85*

ISSN: 1475-2859