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Table 1 Purification of KSI–p53pAnt fused peptide and p53pAnt peptide, overexpressed in E. coli BL21(DE3)

From: Design and implementation of a high yield production system for recombinant expression of peptides

Purification step Total fused peptide (mg) Total p53pAnt (mg) Purity (%) Yield (%)
Total protein extract n.d n.d 43* 100
Inclusion bodies n.d n.d 79* -
First affinity chromatography 184** 43 90* -
Precipitation recovery 184 43 - 100*
Thrombin cleavage - - - 95*
Second affinity chromatography - 34** 95 85*
  1. Data per g of dry cell weight.
  2. Experiments n.d., not determined.
  3. *Determined by SDS-PAGE gels densitometry analysis.
  4. **Determined by Bradford assay.