Table 3 Physiological characteristics of modified strains
Strain | (Over)expressed genes( HIS3 plasmid) & ( URA3 plasmid) | Final FAEE yield (μg gCDW−1) | Maximal specific growth rate (μmax) | Final biomass (OD 600) | Glucose consumption rate (qsmax) | Ethanol consumption rate (qethmax) |
|---|---|---|---|---|---|---|
BdJref | --- & --- | 16.3 ± 3.5 | 0.190 ± 0.010 | 5.84 ± 0.14 | −1.534 ± 0.054 | −0.204 ± 0.015 |
Phosphoketolase pathway | ||||||
BdJ01 | ws2 & --- | 18 ± 4.8 | 0.159 ± 0.006 | 5.51 ± 0.16 | −1.448 ± 0.057 | −0.200 ± 0.021 |
BdJ02 | ws2 & xpkA, ack | 28.0 ± 3.5 | 0.168 ± 0.009 | 5.27 ± 0.13 | −1.357 ± 0.084 | −0.208 ± 0.016 |
BdJ03 | ws2 & xpkA, pta | 105 ± 30 | 0.136 ± 0.025 | 4.55 ± 0.11 | −1.357 ± 0.078 | −0.197 ± 0.014 |
Ethanol degradation pathway | ||||||
BdJ07 | --- & ws2 | 133 ± 113 | 0.192 ± 0.003 | 5.14 ± 0.22 | −1.403 ± 0.035 | −0.174 ± 0.003 |
BdJ08 | ws2, ALD6, ADH2, acs SE L641P & --- | 408 ± 270 | 0.115 ± 0.026 | 3.97 ± 0.42 | −1.265 ± 0.365 | −0.113 ± 0.002 |
BdJ09 | ALD6, ADH2, acs SE L641P & ws2 | 359 ± 128 | 0.136 ± 0.035 | 3.67 ± 1.82 | −1.208 ± 0.495 | −0.061 ± 0.023 |