Table 1 Primers used for PCR construction and amplification of chiA74 ∆s p and chiA74 ∆s p-gfp
Primer | Sequencea |
|---|---|
chiA74-13 | F: 5′-TCCCCGCGG ATG TCACCAAAGCAAAGTCAAAAAATTGTTGGGTAC-3′ |
chiA74-12 | R: 5′-TCCCCGCGG TTCTCCTTTCAAAATAAAAGATATATTTAAAGGC-3′ |
gfp-1 | F: 5′- ATGGCTAGCAAAGGAGAAGAACTTT-3′ |
gfp-3 | R: 5′- GGTCAGATCT TTATTTGTAGAGCTCATCCAT -3′ |
chiA74-C | F: 5′- GGTCAGATCT ACGTAATATCCATTAATTACTTCACTA -3′ |
chiA74-B | R: 5′- GTTTTCGCTAATGACGGCATTTAAAAG -3′ |
cyt-STAB-1 | F: 5′-CGGAATTCTATTTTCGATTTC-3′ |
chiA74-3 | R: 5-AACTGCAG CGAAAGCCTTTCCCTAACAGGTGACTATC-3′ |
ery-1 | F: 5-AAAACTGCAG CTTAAGAGTGTGTTGATAGTGC-3′ |
ery-2 | R: 5-ATAAGAATGCGGCCGC CCCCGTAGGCGCTAGGGACC-3 |