Figure 3
From: 2,2-Diphenyl-1-picrylhydrazyl as a screening tool for recombinant monoterpene biosynthesis
Detection of limonene biosynthesis in a small-scale screen. S. cerevisiae EPY210C expressing the C. limon (CLLS) or M. spicata (MSLS) limonene synthase, or carrying an empty expression vector (Negative) were cultured for 120 h in 5 mL of either SD, YP, or YP + medium (n = 3 biological replicates in each condition). Reaction rates between dodecane extracts and DPPH (100 μM) were calculated and compared to the negative control for each medium composition (mean ± 1 SD, unpaired Student’s t-test, ** = p < 0.01).