Small, synthetic, GC-rich mRNA stem-loop modules 5′ proximal to the AUG start-codon predictably tune gene expression in yeast

Table 3 Effects of growth conditions on Pdr5p, Cdr1p, and Cdr2p expression

^*∆PDR5 = AD; PDR5: wt = AD/PDR5 and S = AD/S-PDR5; CDR1A: Pac I = AD∆/P-CDR1-URA3 and Sfi I-9 = AD∆/construct-9-CDR1; CDR2A: Pac I = AD/P-CDR2A-URA3 and Sfi I = AD/S-CDR2A-URA3 (Table 5).
⁺ Nucleotide changes to the PDR5 5′ UTR just upstream of the ATG start codon.
⁺⁺ Glycerol instead of glucose in the CSM.

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