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Figure 6 | Microbial Cell Factories

Figure 6

From: Small, synthetic, GC-rich mRNA stem-loop modules 5′ proximal to the AUG start-codon predictably tune gene expression in yeast

Figure 6

mRNA secondary structures predicted for the 5′ UTRs of wild-type PDR5 and PDR5 containing the originally created Sfi I stem-loop at −4 (construct #1). The most representative mRNA secondary structures predicted for the entire 5′ UTRs of wild-type PDR5 (three transcription start sites were determined at −171, -174 and −175 [35]) and wild-type PDR5 that has been modified to contain the Sfi I site at −4 (construct #1) are shown in A and B, respectively. Arrows with numbers (kcal/mol) indicate secondary structures of significant stability. 5′ ends are highlighted with black circles and the AUG start-codons are highlighted as grey ovals. The calculated thermodynamic stabilities for the entire 5′ UTR of each predicted secondary structure is shown at the bottom of each structure.

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Microbial Cell Factories

ISSN: 1475-2859

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