p-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans
© Kawai et al.; licensee BioMed Central Ltd. 2013
Received: 26 February 2013
Accepted: 14 April 2013
Published: 7 May 2013
p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmful to the environment. Although pHCA production using microbes has been widely studied, there remains a need for more cost-effective methods, such as the use of biomass as a carbon source. In this study, we produced pHCA using tyrosine ammonia lyase-expressing Streptomyces lividans. In order to improve pHCA productivity from cellulose, we constructed a tyrosine ammonia lyase- and endoglucanase (EG)-expressing S. lividans transformant and used it to produce pHCA from cellulose.
A Streptomyces lividans transformant was constructed to express tyrosine ammonia lyase derived from Rhodobacter sphaeroides (RsTAL). The transformant produced 786 or 736 mg/L of pHCA after 7 days of cultivation in medium containing 1% glucose or cellobiose as the carbon source, respectively. To enhance pHCA production from phosphoric acid swollen cellulose (PASC), we introduced the gene encoding EG into RsTAL-expressing S. lividans. After 7 days of cultivation, this transformant produced 753, 743, or 500 mg/L of pHCA from 1% glucose, cellobiose, or PASC, respectively.
RsTAL-expressing S. lividans can produce pHCA from glucose and cellobiose. Similarly, RsTAL- and EG-expressing S. lividans can produce pHCA from glucose and cellobiose with excess EG activity remaining in the supernatant. This transformant demonstrated improved pHCA production from cellulose. Further enhancements in the cellulose degradation capability of the transformant will be necessary in order to achieve further improvements in pHCA production from cellulose.
KeywordsStreptomyces lividans P-hydroxycinnamic acid Endoglucanase Cellulose
In a world in which fossil fuels are becoming scarcer and more expensive, the production of chemicals from renewable feedstocks represents a promising means of meeting energy demands [1–4]. Considering that the market for aromatics is quite large and the chemical synthesis of aromatics is often cumbersome, the bio-based production of these chemicals from renewable resources would provide a green and economically feasible alternative to current technologies . A number of reports have emerged in recent years concerning the production of aromatic compounds using microbes. For example, Pseudomonas putida has been used to produce p-hydroxystyrene and phenol [6, 7], while p-hydroxybenzoate production was successfully demonstrated using Escherichia coli as a host .
The aromatic compound p-hydroxycinnamic acid (pHCA) serves as the starting compound for the production of numerous commercially valuable chemicals, including flavors, fragrances, pharmaceuticals, biocosmetics, and health and nutrition products , and is also used in the synthesis of thermostable polymers . However, chemical synthesis of pHCA is not cost-effective and results in the production of large amounts of harmful byproducts . Although pHCA production using P. putida, E. coli, or Saccharomyces cerevisiae has been reported [9, 11], technical improvements are needed for industrial production.
Tyrosine ammonia lyase (TAL), a member of the aromatic amino acid lyase family, catalyzes the nonoxidative deamination of L-tyrosine to trans-pHCA . Tyrosine ammonia lyase derived from the oleaginous yeast Rhodotorula glutinis has been used to produce pHCA by introducing R. glutinis TAL into P. putida, E. coli, and S. cerevisiae[9, 11]. In addition, R. glutinis TAL catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. In this study, we utilized TAL derived from the photosynthetic bacterium Rhodobacter sphaeroides (RsTAL) . Because RsTAL demonstrates high substrate specificity for tyrosine, selective production of pHCA is possible.
Cellulose, a β-1, 4-glucose polymer, is the major component of the cell wall of plants and is the most abundant biomass material in the world. The rigid structure of cellulose makes it difficult to degrade into glucose, thus creating a bottleneck in bioprocesses designed to produce useful compounds from cellulose [14, 15]. Three types of cellulolytic enzymes are required for the complete breakdown of cellulose to simple sugars. First, endoglucanase (EG) randomly cleaves glycoside bonds within the interior of the cellulose polymer chain. Exocellobiohydrolase (CBH) then acts progressively on the reducing or nonreducing ends of the cellulose chains, releasing either cellobiose or glucose as the major products. Finally, β-glucosidase (BGL) hydrolyzes the cello-oligosaccharides to glucose . By enhancing the expression levels of these three enzymes, effective microbial bioproduction from cellulose can be achieved.
The Streptomyces are gram-positive, filamentous bacteria known for their ability to produce various antibiotics and secrete heterologous proteins [17, 18]. Streptomyces lividans is the most versatile host within this genus for the production of useful compounds. Pagé et al. demonstrated that genetically modified S. lividans can secrete a large amount of xylanase in culture , while Gamboa-Suasnavart et al. reported production of Mycobacterium tuberculosis APA antigen by S. lividans. Although S. lividans is commonly used for protein production, our previous research involving cinnamic acid production using phenylalanine ammonia lyase-expressing S. lividans also showed that S. lividans is a suitable host for the production of building block compounds , and in the present study we produced pHCA using S. lividans as a host. In our previous report, we demonstrated that while S. lividans does not completely assimilate cellulose, the organism can assimilate cello-oligosaccharides and glucose . To improve the level of pHCA production from cellulose, in the present study we utilized EG, which catalyzes the degradation of cellulose to cello-oligosaccharide.
In this study, we introduced the gene encoding RsTAL into S. lividans, and pHCA was produced from glucose or cellobiose using S. lividans/pURsTAL. The production of pHCA when cellobiose served as the carbon source was equal to that obtained with glucose as the carbon source; however, the amount of pHCA produced from cellulose was extremely low. In order to enhance pHCA productivity using cellulose as the carbon source, we therefore constructed an EG- and RsTAL-expressing S. lividans strain, and were able to demonstrate for the first time effective production of pHCA directly from cellulose.
Results and discussion
Production of pHCA by S. lividans/pURsTAL
A UV analysis of the putative pHCA HPLC fraction isolated from the culture supernatant of S. lividans/pURsTAL revealed the presence of three prominent absorption peaks in the 200–350 nm region that were consistent with the peaks produced upon analysis of standard pHCA (Figure 1B). In addition, we carried out pHCA production using S. lividans/pURsTAL using the modified TSB medium with 1% glucose and the additional L-tyrosine, which is the precursor of pHCA. The addition of L-tyrosine into the initial culture medium increased the peak areas of pHCA (data not shown). These results confirmed that introduction of the gene encoding RsTAL into S. lividans enables the production of pHCA.
We also examined pHCA production by the control, S. lividans/pU. As expected, this strain did not produce any pHCA (data not shown). Figure 2B shows the change in the dry cell weight over time of S. lividans/pURsTAL cultured in medium containing 1% glucose or cellobiose. The growth of S. lividans/pURsTAL in medium containing 1% cellobiose as the carbon source was almost the same as in cultures in which 1% glucose served as the carbon source, indicating that S. lividans can assimilate cellobiose and produce pHCA as well as utilize glucose, in agreement with our previous report . Here, we estimated the amount of residual glucose or cellobiose using HPLC. In the case of each transformant, glucose or cellobiose added to the medium was consumed within 2 days (data not shown). These results strongly suggested that S. lividans/pURsTAL could assimilate cellobiose as well as glucose.
Construction of EG- and RsTAL-expressing S. lividans
Most microorganisms have difficulty degrading cellulose due to its rigid structure. In order to develop effective microbial bioprocesses utilizing cellulose as a carbon source, it is therefore necessary to introduce multiple genes encoding cellulose degradation enzymes into the genome of the organism of interest. A recent report described the introduction of four types of cellulases into Saccharomyces cerevisiae and subsequent bioethanol production from cellulose . Bokinsky et al. reported the use of E. coli expressing three types of cellulases for the production of advanced biofuels from cellulose .
Streptomyces lividans constitutively expresses a highly active form of BGL that enables the organism to assimilate cello-oligosaccharide . However, the ability of S. lividans to degrade cellulose must be improved in order to achieve effective bioconversion of cellulose to useful compounds. In this study, we demonstrated production of pHCA using cellobiose as the carbon source. To achieve effective pHCA production from cellulose, we then introduced the gene encoding EG into pHCA-producing S. lividans in order to facilitate the degradation of cellulose to cello-oligosaccharide. For this purpose we chose Tfu0901, which is a highly active EG derived from T. fusca YX, and introduced the gene for this enzyme into RsTAL-expressing S. lividans. After the Tfu0901 gene was introduced into wild-type S. lividans using the integration type vector pTYM18 , the resulting S. lividans mutant was able to produce pHCA after introduction of the multicopy type vector pUC702 that carries the gene encoding RsTAL. The resulting EG- and RsTAL-expressing S. lividans strain was designated S. lividans/pT09pURsTAL.
Production of pHCA directly from cellulose by EG- and RsTAL-expressing S. lividans
Using S. lividans/pT09pURsTAL, we produced pHCA from the cellulosic substrate phosphoric acid swollen cellulose (PASC). Figure 3A shows the time course of pHCA production in modified TSB medium containing 1% PASC as the carbon source. The maximal level of pHCA production reached by S. lividans/pT09pURsTAL was 500 mg/L after 7 days of cultivation, whereas the control strain, S. lividans/pURsTAL, produced 310 mg/L after 7 days of cultivation. The change in supernatant EG activity over time with 1% PASC serving as the carbon source is shown in Figure 3B. The maximal level of EG activity in the supernatant of S. lividans/pT09pURsTAL was approximately 210 U/L, whereas that of S. lividans/pURsTAL was only 10 U/L. Although S. lividans/pURsTAL produced some quantity of pHCA from cellulose, S. lividans is known to secrete several kinds of cellulases, including EG, and thus this result is consistent with those of our previous report .
In the present study, production of pHCA from cellulose was improved by using the S. lividans/pT09pURsTAL strain, which produced 500 mg/L of pHCA, a level that was 1.6-fold higher than the level of pHCA produced by S. lividans/pURsTAL. Production of pHCA was carried out using S. lividans/pT09pURsTAL cultured with 1% cellobiose as the carbon source. Figure 3A shows the production of pHCA over time in modified TSB medium containing 1% cellobiose as the carbon source. The maximal level of pHCA produced by S. lividans/pT09pURsTAL from 1% cellobiose was 743 mg/L after 7 days of cultivation. This level was slightly higher than that produced from 1% PASC (Figure 3A). These results indicate that S. lividans/pT09pURsTAL did not completely assimilate the 1% PASC. The amount of pHCA produced by S. lividans/pT09pURsTAL from 1% cellobiose was equal to that produced from 1% glucose, and the introduction of the gene encoding EG did not affect the ability of the organism to assimilate cellobiose. Hence, to produce pHCA from cellulose more effectively, the efficiency of the cellulose to cello-oligosaccharide degradation reaction should be improved.
Due to synergism between EG and CBH, cellulose is effectively degraded to cello-oligosaccharide, which is a suitable carbon source for S. lividans[22, 23]. To enhance pHCA productivity from cellulose, in this study we utilized an EG derived from T. fusca YX, Tfu0901, to construct EG- and RsTAL-expressing S. lividans/pT09pURsTAL, which was capable of producing pHCA from PASC (Figure 3A) due to the increased availability of cello-oligosaccharides relative to the control strain. However, the amount of pHCA produced from 1% PASC was lower than that produced from 1% glucose or cellobiose (Figure 3A), indicating that PASC was not completely degraded to available sugars. One promising means of achieving higher pHCA productivity from PASC would be to introduce the gene encoding CBH into S. lividans/pT09pURsTAL. Exocellobiohydrolase can act on the reducing or nonreducing ends of cellulose chains generated by EG, leading to pHCA production comparable to that obtained when either glucose or cellobiose is employed as the carbon source. Currently, our group is screening for active CBH and developing a three-gene expression system for S. lividans.
We demonstrated the production of pHCA from glucose and cellobiose as carbon sources using RsTAL-expressing S. lividans. The amount of pHCA produced in batch culture using our system was higher than that reported in previous studies [9, 11]. In order to improve the production of pHCA from cellulose, we constructed a strain of S. lividans that expresses both EG and RsTAL. This transformant could secrete EG into the supernatant and produce pHCA directly from cellulose. Our results demonstrate that S. lividans can be used as a host to produce aromatic building-blocks from cellulose.
Strains, plasmids, transformants and oligonucleotide primers used in this study
Strain, plasmid, primer, or transformant
Relevant features (5′-3′)
Source or reference
endA1 hsdR17(r K12 - m K12 +) supE44 thi-I gyrA96 relA1 lac recA1/F′[proAB + lacIq ZΔM15::Tn10(Tetr)]
TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir
Streptomyces lividans 1326
WT strain (NBRC 15675)
Versatile vector for protein expression; thiostrepton resistance marker
Noda et al. 2010
Vector for RsTAL expression; thiostrepton resistance marker
Intergeneric conjugation vector; kanamycin resistance marker
Onaka et al. 2003
Versatile integration vector for protein expression; kanamycin resistance marker
Vector for secreting endoglucanase (Tfu0901); kanamycin resistance marker
Transformant harboring pUC702
Transformant harboring pUC702-p-RsTAL
Transformant integrating pTYM18-ps-Tfu0901 and harboring pUC702-p-RsTAL
The integration-type vector for the expression of EG was constructed as follows. First, pUC702-pro-sig-term was digested with Hind III and Kpn I, and the resulting fragment encoding the promoter, signal sequence, and terminator was subcloned into the Hind III and Kpn I restriction sites of pTYM18 [24, 25], which served as a shuttle vector between E. coli and S. lividans. The resulting plasmid was designated pTYM18-pro-sig-term. The gene fragments encoding Tfu0901 were amplified by PCR using the T. fusca YX genome (ATCC 27730) as a template with the Tfu0901I_F and Tfu0901I_R primers. The Tfu0901 fragment was digested with Nhe I and Bam HI and introduced into the Nhe I and Bam HI restriction sites of pTYM18-pro-sig-term.
Bacterial strains, transformation, and cultivation
The strains and transformants used in this study are summarized in Table 1. The integration-type plasmid pTYM18-ps-Tfu0901 was constructed and transformed into E. coli S17-1 λpir (BIOMEDAL, Seville, Spain). A single transformant colony was picked and cultivated at 37°C for 8 h in 5 mL of LB medium containing 40 μg/mL of kanamycin. The cells were harvested and washed three times with LB broth to remove the kanamycin. Next, the cells were suspended in 500 μL of LB medium and mixed with wild-type S. lividans spores. The mixture was plated on ISP4 medium (1.0% soluble starch, 0.1% K2HPO4, 0.1% MgSO4 · 7 H2O, 0.1% NaCl, 0.2% (NH4)2SO4, 0.2% CaCO3, 0.0001% FeSO4, 0.0001% MnCl2, 0.0001% ZnSO4, and 2.0% agar) and incubated for 18 h at 30°C. A 3-mL aliquot of soft-agar nutrient broth containing kanamycin (50 μg/mL) and nalidixic acid (67 μg/mL) was dispensed in layers onto the plate, which was then incubated at 30°C for 5 days, after which a single colony was picked and streaked onto ISP4 agar containing kanamycin (50 μg/mL) and nalidixic acid (5 μg/mL) and incubated at 30°C for 5 days for screening.
Protplasts of wild-type S. lividans 1326 and S. lividans/pT09 were prepared according to the method of Hopwood et al. . Briefly, mycelia of each strain were treated with 1 mg/mL of lysozyme solution (Wako, Osaka, Japan), and the suspended mycelia were used as protoplasts. The plasmid pUC702-p-RsTAL was introduced into wild-type S. lividans using the polyethylene glycol method. The pUC702-p-RsTAL plasmid was also introduced into S. lividans/pT09. Selection of transformants was carried out by overlaying soft agar containing 50 μg/mL of thiostrepton, or 50 μg/mL of thiostrepton and kanamycin.
To produce pHCA, a single colony of either S. lividans/pURsTAL or S. lividans/pT09pURsTAL was inoculated into a test tube containing 5 mL of TSB medium supplemented with 5 μg/mL of thiostrepton or 5 μg/mL of thiostrepton and 50 μg/mL of kanamycin, followed by cultivation at 28°C for 3 days. Next, 5 mL of S. lividans/pURsTAL or S. lividans/pT09pURsTAL preculture medium was seeded into a shake flask equipped with a baffle and containing 100 mL of modified TSB medium with 1% glucose, cellobiose, or PASC as a carbon source, and the flask was incubated at 28°C for 7 days. In this study, modified TSB medium consisted of 3% TSB with 5% tryptone as the nitrogen source.
Measurement of EG activity
Endoglucanase activity was measured according to a method established by Miller , with some modifications. Briefly, a 300-μL aliquot of culture supernatant was mixed with 700 μL of a 1% (w/v) solution of carboxymethylcellulose (CMC) dissolved in 50 mM sodium acetate buffer (pH 7.0) and the mixture was incubated at 50°C for 1 h. The amount of reducing sugar released from the CMC was assessed by determining the amount of glucose and equivalents present using the dinitrosalicylic acid method. One unit of enzyme activity was defined as the amount of enzyme required to release 1 μmol of reducing sugar as glucose, for CMC, and equivalents, from the substrate, per min.
The pHCA concentration was determined by high-performance liquid chromatography (HPLC; Shimadzu, Kyoto, Japan) using a Cholester column (Nacalai Tesque, Kyoto, Japan). The HPLC column was maintained at 30°C and eluted at a flow rate of 1.2 mL/min. A dual solvent system was used. Solvent A was phosphate buffer (50 mM, pH 2.0) and solvent B was acetonitrile. The gradient began at 80% solvent A and 20% solvent B, and a 50–50 mixture of solvents A and B was employed from 12 to 17 min. From 17.01 to 20.00 min the solvent composition was 80% solvent A and 20% solvent B.
The concentration of pHCA was determined using an ultraviolet absorbance detector (SPD-20AV, Shimadzu). Culture supernatant was obtained by centrifuging the culture broth at 21,880 × g for 20 min, and was followed by HPLC analysis of the supernatant. A JASCO V-650 spectrophotometer (JASCO Corporation, Tokyo, Japan) was used to collect UV absorption spectra.
This work was supported by Special Coordination Funds for Promoting Science and Technology, provided by the Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovation Bioproduction Kobe), MEXT, Japan.
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