Table 2 Plasmids used in this study
Plasmid | Description | Source or reference |
|---|---|---|
pGK423 | Yeast expression vector containing PGK1 promoter, 2 μ origin, HIS3 marker, no expression (control plasmid) | Ishii et al., 2009 [34] |
pGK425 | Yeast expression vector containing PGK1 promoter, 2 μ origin, LEU2 marker, no expression (control plasmid) | Ishii et al., 2009 [34] |
pGK426 | Yeast expression vector containing PGK1 promoter, 2 μ origin, URA3 marker, no expression (control plasmid) | Ishii et al., 2009 [34] |
pATP423 | Yeast three gene expression vector containing ADH1, TDH3 and PGK1 promoters, 2 μ origin, HIS3 marker, no expression (control plasmid) | Ishii et al., in submission |
pATP425 | Yeast three gene expression vector containing ADH1, TDH3, and PGK1 promoters, 2 μ origin, LEU2 marker, no expression (control plasmid) | Ishii et al., in submission |
pATP426 | Yeast three gene expression vector containing ADH1, TDH3, and PGK1 promoters, 2 μ origin, URA3 marker, no expression (control plasmid) | Ishii et al., in submission |
pGK423-kivd | pGK423, expression of L. lactis 2-ketoisovalerate decarboxylase (kivd) gene | Kondo et al., 2012 [17] |
pGK425-ILV2 | pGK425, expression of S. cerevisiae ILV2 gene | Kondo et al., 2012 [17] |
pGK426-ADH6 | pGK426, expression of S. cerevisiae ADH6 gene | Kondo et al., 2012 [17] |
pILV532cytL | pATP425, co-expression of S. cerevisiae ILV5c, ILV3c, and ILV2c genes | This study |
pILV532cytM | 2 μ origin, MET15 marker (pGK421-base), co-expression of S. cerevisiae ILV5c, ILV3c, and ILV2c genes | This study |
pATP423-sMAE1 | pATP423, expression of S. cerevisiae sMAE1 gene | This study |
pATP423-MsM | pATP423, co-expression of S. cerevisiae sMAE1 and MDH2 genes | This study |
pATP423-PMsM | pATP423, co-expression of S. cerevisiae sMAE1, MDH2, and PYC2 genes | This study |
pATP423-MAE1 | pATP423, expression of S. cerevisiae MAE1 gene | This study |
pATP423-MM | pATP423, co-expression of S. cerevisiae MAE1 and MDH2 genes | This study |
pATP423-PMM | pATP423, co-expression of S. cerevisiae MAE1, MDH2, and PYC2 genes | This study |
pILV2L | pATP425, expression of S. cerevisiae ILV2 gene | This study |
pATP426-kivd-ADH6 | pATP426, co-expression of L. lactis kivd and S. cerevisiae ADH6 genes | This study |
pATP426-kivd-ADH6-ILV2 | pATP426, co-expression of L. lactis kivd, S. cerevisiae ADH6, and ILV2 genes | This study |