Table 2 Primer sequences
From: Isobutyraldehyde production from Escherichia coli by removing aldehyde reductase activity
Primer | Sequence 5’ → 3’ |
|---|---|
GR180 | TCTAGAGGCATCAAATAAAACGAAAGGCTC |
GR181 | GGTATATCTCCTGCATGCTTATGATTTATTTTG |
GR219 | GCATGCAGGAGAAAGGTCACatgaaggctgcagttgttacgaagg |
GR220 | TTTTATTTGATGCCTCTAGAttagtgacggaaatcaatcaccatg |
GR221 | GCATGCAGGAGAAAGGTCACatgcaaaatgaattgcagaccgcgc |
GR222 | TTTTATTTGATGCCTCTAGAttattgcgccgctgcgtacaggccg |
GR223 | GCATGCAGGAGAAAGGTCACatggcagcttcaacgttctttattc |
GR224 | TTTTATTTGATGCCTCTAGAttacatcgctgcgcgataaatcgcc |
GR225 | GCATGCAGGAGAAAGGTCACatgtcgatgataaaaagctatgccg |
GR226 | TTTTATTTGATGCCTCTAGAtcaaaaatcggctttcaacaccacg |
GR227 | GCATGCAGGAGAAAGGTCACAtgcaatttgactacatcattattg |
GR228 | TTTTATTTGATGCCTCTAGAtcattttttcgctctcaccggcatc |
GR229 | GCATGCAGGAGAAAGGTCACatgatggctaacagaatgattctga |
GR230 | TTTTATTTGATGCCTCTAGAttaccaggcggtatggtaaagctct |
GR231 | GCATGCAGGAGAAAGGTCACatgaatcaacaggatattgaacagg |
GR232 | TTTTATTTGATGCCTCTAGAttaaacaatgcgaaacgcatcgact |
GR145 | GGTACCTTTCTCCTCTTTAATGAA |
GR146 | TAATGACTCTAGAGGCATCAAATAA |
GR147 | ATTCATTAAAGAGGAGAAAGGTACCatgaaggctgcagttgttacgaagg |
GR148 | TTATTTGATGCCTCTAGAGTCATTAttagtgacggaaatcaatcaccatg |
GR149 | ATTCATTAAAGAGGAGAAAGGTACCatgcaaaatgaattgcagaccgcgc |
GR150 | TTATTTGATGCCTCTAGAGTCATTAttattgcgccgctgcgtacaggccg |
GR151 | ATTCATTAAAGAGGAGAAAGGTACCatggcagcttcaacgttctttattc |
GR152 | TTATTTGATGCCTCTAGAGTCATTAttacatcgctgcgcgataaatcgcc |
GR153 | ATTCATTAAAGAGGAGAAAGGTACCatgtcgatgataaaaagctatgccg |
GR154 | TTATTTGATGCCTCTAGAGTCATTAtcaaaaatcggctttcaacaccacg |
GR188 | ATTCATTAAAGAGGAGAAAGGTACCAtgcaatttgactacatcattattggtg |
GR189 | TTATTTGATGCCTCTAGAGTCATTAtcattttttcgctctcaccggcatc |
GR190 | ATTCATTAAAGAGGAGAAAGGTACCatgatggctaacagaatgattctgaac |
GR191 | TTATTTGATGCCTCTAGAGTCATTAttaccaggcggtatggtaaagctc |
GR192 | ATTCATTAAAGAGGAGAAAGGTACCatgaatcaacaggatattgaacaggtg |
GR193 | TTATTTGATGCCTCTAGAGTCATTAttaaacaatgcgaaacgcatcgact |