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Figure 2 | Microbial Cell Factories

Figure 2

From: Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes

Figure 2

CYP activity and Y. lipolytica biomass production during induction. CYP2D6 (1’-hydroxybufuralol formation, A) and CYP3A4 (6β-hydroxyprogesterone formation, B) activity during ethanol induction of Y. lipolytica strains YL21 and YL22, respectively. The cultures were incubated at 28°C and induced by ethanol after glucose depletion. The Roman numerals indicate the following process phases: I) batch growth with glucose; II) induction phase of about 70 h. Squares highlight biomass concentrations determined as cell dry weight, while bars document CYP expression levels measured as specific whole-cell activity. Experiments were performed in triplicates. Samples were taken after 4,8,21,26,31,45,50,55 and 69 h.

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Microbial Cell Factories

ISSN: 1475-2859

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