Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum

Table 3 Ratio of expression levels of sppKR versus lacLM in L. plantarum WCFS1 carrying pEH9R and L. plantarum WCFS1 carrying pEH9A

Time (h)	Time after induction (h)	pEH9R	pEH9A
6^a	0	4.87 ± 0.78	14.32 ± 2.96
8	2	0.15 ± 0.01	2.01 ± 0.03
12	6	0.15 ± 0.03	2.40 ± 0.23

Values were obtained by dividing the average transcript number of sppKR with the average transcript number of lacLM as described in Material and Methods
^a Just before induction

ISSN: 1475-2859