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  1. Research

    Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

    Recombinant expression of toxic proteins remains a challenging problem. One potential method to shield toxicity and thus improve expression of these proteins is to encapsulate them within protein compartments ...

    Mimi C. Yung, Feliza A. Bourguet, Timothy S. Carpenter and Matthew A. Coleman

    Microbial Cell Factories 2017 16:71

    Published on: 26 April 2017

  2. Research

    Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

    The exploitation of the CRISPR/Cas9 machinery coupled to lambda (λ) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli. First proposed by...

    Francesca Zerbini, Ilaria Zanella, Davide Fraccascia, Enrico König, Carmela Irene, Luca F. Frattini, Michele Tomasi, Laura Fantappiè, Luisa Ganfini, Elena Caproni, Matteo Parri, Alberto Grandi and Guido Grandi

    Microbial Cell Factories 2017 16:68

    Published on: 24 April 2017

  3. Research

    A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis

    Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a centr...

    Dongbo Cai, Hao Wang, Penghui He, Chengjun Zhu, Qin Wang, Xuetuan Wei, Christopher T. Nomura and Shouwen Chen

    Microbial Cell Factories 2017 16:70

    Published on: 24 April 2017

  4. Research

    Inheritance of brewing-relevant phenotypes in constructed Saccharomyces cerevisiae × Saccharomyces eubayanus hybrids

    Interspecific hybridization has proven to be a potentially valuable technique for generating de novo lager yeast strains that possess diverse and improved traits compared to their parent strains. To further en...

    Kristoffer Krogerus, Tuulikki Seppänen-Laakso, Sandra Castillo and Brian Gibson

    Microbial Cell Factories 2017 16:66

    Published on: 21 April 2017

  5. Research

    Genomics insights into different cellobiose hydrolysis activities in two Trichoderma hamatum strains

    Efficient biomass bioconversion is a promising solution to alternative energy resources and environmental issues associated with lignocellulosic wastes. The Trichoderma species of cellulolytic fungi have strong c...

    Peng Cheng, Bo Liu, Yi Su, Yao Hu, Yahui Hong, Xinxin Yi, Lei Chen, Shengying Su, Jeffrey S. C. Chu, Nansheng Chen and Xingyao Xiong

    Microbial Cell Factories 2017 16:63

    Published on: 19 April 2017

  6. Review

    Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery

    Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves as a sensor, and a response regula...

    Sambandam Ravikumar, Mary Grace Baylon, Si Jae Park and Jong-il Choi

    Microbial Cell Factories 2017 16:62

    Published on: 14 April 2017

  7. Review

    Gas fermentation: cellular engineering possibilities and scale up

    Low carbon fuels and chemicals can be sourced from renewable materials such as biomass or from industrial and municipal waste streams. Gasification of these materials allows all of the carbon to become availab...

    Björn D. Heijstra, Ching Leang and Alex Juminaga

    Microbial Cell Factories 2017 16:60

    Published on: 12 April 2017

  8. Research

    Accelerated triacylglycerol production and altered fatty acid composition in oleaginous microalga Neochloris oleoabundans by overexpression of diacylglycerol acyltransferase 2

    Microalgae are promising sources of lipid triacylglycerol (TAG) for biodiesel production. However, to date, microalgal biodiesel is technically feasible, but not yet economically viable. Increasing TAG content...

    Paeka Klaitong, Sirirat Fa-aroonsawat and Wipa Chungjatupornchai

    Microbial Cell Factories 2017 16:61

    Published on: 12 April 2017

  9. Research

    Improved curdlan production with discarded bottom parts of Asparagus spear

    This work evaluated the improvement of curdlan production of Agrobacterium sp. ATCC 31749 by using culture medium containing juice of discarded bottom part of green Asparagus spear (MJDA). Curdlan production was ...

    Rex Frimpong Anane, Huifang Sun, Lamei Zhao, Le Wang, Chun Lin and Zichao Mao

    Microbial Cell Factories 2017 16:59

    Published on: 7 April 2017

  10. Research

    Intramembrane protease RasP boosts protein production in Bacillus

    The microbial cell factory Bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. Nonetheless, the effective secretion of particular heterologous enzymes remai...

    Jolanda Neef, Cristina Bongiorni, Vivianne J. Goosens, Brian Schmidt and Jan Maarten van Dijl

    Microbial Cell Factories 2017 16:57

    Published on: 4 April 2017

  11. Review

    A review on Lactococcus lactis: from food to factory

    Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the...

    Adelene Ai-Lian Song, Lionel L. A. In, Swee Hua Erin Lim and Raha Abdul Rahim

    Microbial Cell Factories 2017 16:55

    Published on: 4 April 2017

  12. Research

    Heterologous biosynthesis and manipulation of crocetin in Saccharomyces cerevisiae

    Due to excellent performance in antitumor, antioxidation, antihypertension, antiatherosclerotic and antidepressant activities, crocetin, naturally exists in Crocus sativus L., has great potential applications in ...

    Fenghua Chai, Ying Wang, Xueang Mei, Mingdong Yao, Yan Chen, Hong Liu, Wenhai Xiao and Yingjin Yuan

    Microbial Cell Factories 2017 16:54

    Published on: 29 March 2017

  13. Research

    Expanding metabolic pathway for de novo biosynthesis of the chiral pharmaceutical intermediate l-pipecolic acid in Escherichia coli

    The six-carbon circular non-proteinogenic compound l-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolica...

    Hanxiao Ying, Sha Tao, Jing Wang, Weichao Ma, Kequan Chen, Xin Wang and Pingkai Ouyang

    Microbial Cell Factories 2017 16:52

    Published on: 27 March 2017

  14. Research

    Application of an E. coli signal sequence as a versatile inclusion body tag

    Heterologous protein production in Escherichia coli often suffers from bottlenecks such as proteolytic degradation, complex purification procedures and toxicity towards the expression host. Production of proteins...

    Wouter S. P. Jong, David Vikström, Diane Houben, H. Bart van den Berg van Saparoea, Jan-Willem de Gier and Joen Luirink

    Microbial Cell Factories 2017 16:50

    Published on: 21 March 2017

  15. Research

    Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

    Pichia pastoris is a widely used eukaryotic expression host for recombinant protein production. Adaptive laboratory evolution (ALE) has been applied in a wide range of studies in order...

    Josef W. Moser, Roland Prielhofer, Samuel M. Gerner, Alexandra B. Graf, Iain B. H. Wilson, Diethard Mattanovich and Martin Dragosits

    Microbial Cell Factories 2017 16:49

    Published on: 17 March 2017

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