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  1. Research

    Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases

    γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding o...

    Yao Zhang, Xiao Luan, Huaiyuan Zhang, Victoriano Garre, Yuanda Song and Colin Ratledge

    Microbial Cell Factories 2017 16:113

    Published on: 21 June 2017

  2. Research

    Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media

    The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in t...

    Anna Gąciarz, Narendar Kumar Khatri, M. Lourdes Velez-Suberbie, Mirva J. Saaranen, Yuko Uchida, Eli Keshavarz-Moore and Lloyd W. Ruddock

    Microbial Cell Factories 2017 16:108

    Published on: 15 June 2017

  3. Research

    An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli

    The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light-driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired pheno...

    Hyun Aaron Kim, Hyun Ju Kim, Jihoon Park, Ah Reum Choi, Kyoo Heo, Haeyoung Jeong, Kwang-Hwan Jung, Yeong-Jae Seok, Pil Kim and Sang Jun Lee

    Microbial Cell Factories 2017 16:111

    Published on: 15 June 2017

  4. Research

    Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae

    Phaseolamin or α-amylase inhibitor 1 (αAI) is a glycoprotein from common beans (Phaseolus vulgaris L.) that inhibits some insect and mammalian α-amylases. Several clinical studies support the beneficial use of be...

    Stephanie Brain-Isasi, Alejandro Álvarez-Lueje and Thomas Joseph V. Higgins

    Microbial Cell Factories 2017 16:110

    Published on: 15 June 2017

  5. Review

    Whole-cell biocatalysts by design

    Whole-cell biocatalysts provide unique advantages and have been widely used for the efficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients. What is more, ...

    Baixue Lin and Yong Tao

    Microbial Cell Factories 2017 16:106

    Published on: 13 June 2017

  6. Research

    Three steps in one pot: biosynthesis of 4-hydroxycinnamyl alcohols using immobilized whole cells of two genetically engineered Escherichia coli strains

    4-Hydroxycinnamyl alcohols are a class of natural plant secondary metabolites that include p-coumaryl alcohol, caffeyl alcohol, coniferyl alcohol and sinapyl alcohol, and have physiological, ecological and biomed...

    Shuxin Liu, Jiabin Liu, Jiayin Hou, Nan Chao, Ying Gai and Xiangning Jiang

    Microbial Cell Factories 2017 16:104

    Published on: 12 June 2017

  7. Research

    Heterologous expression of cytotoxic sesquiterpenoids from the medicinal mushroom Lignosus rhinocerotis in yeast

    Genome mining facilitated by heterologous systems is an emerging approach to access the chemical diversity encoded in basidiomycete genomes. In this study, three sesquiterpene synthase genes, GME3634, GME3638,...

    Hui-Yeng Yeannie Yap, Mariano Jordi Muria-Gonzalez, Boon-Hong Kong, Keith A. Stubbs, Chon-Seng Tan, Szu-Ting Ng, Nget-Hong Tan, Peter S. Solomon, Shin-Yee Fung and Yit-Heng Chooi

    Microbial Cell Factories 2017 16:103

    Published on: 12 June 2017

  8. Research

    Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster

    Nocamycins I and II, produced by the rare actinomycete Saccharothrix syringae, belong to the tetramic acid family natural products. Nocamycins show potent antimicrobial activity and they hold great potential for ...

    Xuhua Mo, Chunrong Shi, Chun Gui, Yanjiao Zhang, Jianhua Ju and Qingji Wang

    Microbial Cell Factories 2017 16:100

    Published on: 9 June 2017

  9. Research

    Microtiter plate cultivation of oleaginous fungi and monitoring of lipogenesis by high-throughput FTIR spectroscopy

    Oleaginous fungi can accumulate lipids by utilizing a wide range of waste substrates. They are an important source for the industrial production of omega-6 polyunsaturated fatty acids (gamma-linolenic and arac...

    Gergely Kosa, Achim Kohler, Valeria Tafintseva, Boris Zimmermann, Kristin Forfang, Nils Kristian Afseth, Dimitrios Tzimorotas, Kiira S. Vuoristo, Svein Jarle Horn, Jerome Mounier and Volha Shapaval

    Microbial Cell Factories 2017 16:101

    Published on: 9 June 2017

  10. Technical notes

    Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker

    A commonly used approach to improve recombinant protein production is to increase the levels of expression by providing extra-copies of a heterologous gene. In Komagataella phaffii (Pichia pastoris) this is usual...

    Maritza Ocampo Betancur, Viviane Castelo Branco Reis, André Moraes Nicola, Janice Lisboa De Marco, Lídia Maria Pepe de Moraes and Fernando Araripe Gonçalves Torres

    Microbial Cell Factories 2017 16:99

    Published on: 8 June 2017

  11. Research

    Construction of energy-conserving sucrose utilization pathways for improving poly-γ-glutamic acid production in Bacillus amyloliquefaciens

    Sucrose is an naturally abundant and easily fermentable feedstock for various biochemical production processes. By now, several sucrose utilization pathways have been identified and characterized. Among them, ...

    Jun Feng, Yanyan Gu, Yufen Quan, Weixia Gao, Yulei Dang, Mingfeng Cao, Xiaoyun Lu, Yi Wang, Cunjiang Song and Shufang Wang

    Microbial Cell Factories 2017 16:98

    Published on: 6 June 2017

  12. Research

    Construct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine

    The sexual stages of Plasmodium falciparum are responsible for the spread of the parasite in malaria endemic areas. The cysteine-rich Pfs48/45 protein, exposed on the surface of sexual stages, is one of the most ...

    Susheel K. Singh, Will Roeffen, Ulrik H. Mistarz, Bishwanath Kumar Chourasia, Fen Yang, Kasper D. Rand, Robert W. Sauerwein and Michael Theisen

    Microbial Cell Factories 2017 16:97

    Published on: 31 May 2017

  13. Research

    Expression of deleted, atoxic atypical recombinant beta2 toxin in a baculovirus system and production of polyclonal and monoclonal antibodies

    Clostridium perfringens is an important animal and human pathogen that can produce more than 16 different major and minor toxins. The beta-2 minor toxin (CPB2), comprising atypical and...

    Anna Serroni, Chiara Francesca Magistrali, Giovanni Pezzotti, Luca Bano, Martina Pellegrini, Giulio Severi, Chiara Di Pancrazio, Mirella Luciani, Manuela Tittarelli, Silvia Tofani and Antonio De Giuseppe

    Microbial Cell Factories 2017 16:94

    Published on: 25 May 2017

  14. Research

    A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis

    Microbial secondary metabolism is regulated by a complex and mostly-unknown network of global and pathway-specific regulators. A dozen biosynthetic gene clusters for secondary metabolites have been reported in...

    Xin-jing Yue, Xiao-wen Cui, Zheng Zhang, Ran Peng, Peng Zhang, Zhi-feng Li and Yue-zhong Li

    Microbial Cell Factories 2017 16:92

    Published on: 23 May 2017

  15. Research

    Effect of bacteria type and sucrose concentration on levan yield and its molecular weight

    Levan has been traditionally produced from microorganism. However, there is a continuous effort in looking for new strains that improve levan production yield and uses alternative sugar sources for growth. Des...

    Álvaro González-Garcinuño, Antonio Tabernero, José Mª Sánchez-Álvarez, Miguel A. Galán and Eva M. Martin del Valle

    Microbial Cell Factories 2017 16:91

    Published on: 23 May 2017

  16. Research

    Intracellular product recycling in high succinic acid producing yeast at low pH

    The metabolic engineering of Saccharomyces cerevisiae for the production of succinic acid has progressed dramatically, and a series of high-producing hosts are available. At low cultivation pH and high titers, th...

    S. Aljoscha Wahl, Cristina Bernal Martinez, Zheng Zhao, Walter M. van Gulik and Mickel L. A. Jansen

    Microbial Cell Factories 2017 16:90

    Published on: 23 May 2017

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