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  1. Research

    Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter

    Baker’s yeast Saccharomyces cerevisiae is a proven host for the commercial production of recombinant biopharmaceutical proteins. For the manufacture of heterologous proteins with activities deleterious to the hos...

    Thea S. B. Møller, Joanna Hay, Malcolm J. Saxton, Karen Bunting, Evamaria I. Petersen, Søren Kjærulff and Christopher J. A. Finnis

    Microbial Cell Factories 2017 16:11

    Published on: 18 January 2017

  2. Research

    Engineered biosynthesis of milbemycins in the avermectin high-producing strain Streptomyces avermitilis

    Milbemycins, produced from Streptomyces hygroscopicus subsp. aureolacrimosus and Streptomyces bingchenggensis, are 16-membered macrolides that share structural similarity with avermectin produced from Streptomyce...

    Myoun-Su Kim, Wan-Je Cho, Myoung Chong Song, Seong-Whan Park, Kaeun Kim, Eunji Kim, Naryeong Lee, Sang-Jip Nam, Ki-Hoon Oh and Yeo Joon Yoon

    Microbial Cell Factories 2017 16:9

    Published on: 17 January 2017

  3. Research

    Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

    Animal venoms are large, complex libraries of bioactive, disulphide-rich peptides. These peptides, and their novel biological activities, are of increasing pharmacological and therapeutic importance. However, ...

    Ana Filipa Sequeira, Jeremy Turchetto, Natalie J. Saez, Fanny Peysson, Laurie Ramond, Yoan Duhoo, Marilyne Blémont, Vânia O. Fernandes, Luís T. Gama, Luís M. A. Ferreira, Catarina I. P. I. Guerreiro, Nicolas Gilles, Hervé Darbon, Carlos M. G. A. Fontes and Renaud Vincentelli

    Microbial Cell Factories 2017 16:4

    Published on: 17 January 2017

  4. Research

    CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

    Anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins ...

    Brady F. Cress, Quentin D. Leitz, Daniel C. Kim, Teresita D. Amore, Jon Y. Suzuki, Robert J. Linhardt and Mattheos A. G. Koffas

    Microbial Cell Factories 2017 16:10

    Published on: 17 January 2017

  5. Research

    High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery

    Animal venoms are complex molecular cocktails containing a wide range of biologically active disulphide-reticulated peptides that target, with high selectivity and efficacy, a variety of membrane receptors. Di...

    Jeremy Turchetto, Ana Filipa Sequeira, Laurie Ramond, Fanny Peysson, Joana L. A. Brás, Natalie J. Saez, Yoan Duhoo, Marilyne Blémont, Catarina I. P. D. Guerreiro, Loic Quinton, Edwin De Pauw, Nicolas Gilles, Hervé Darbon, Carlos M. G. A. Fontes and Renaud Vincentelli

    Microbial Cell Factories 2017 16:6

    Published on: 17 January 2017

  6. Research

    Improvement of yeast tolerance to acetic acid through Haa1 transcription factor engineering: towards the underlying mechanisms

    Besides being a major regulator of the response to acetic acid in Saccharomyces cerevisiae, the transcription factor Haa1 is an important determinant of the tolerance to this acid. The engineering of Haa1 either ...

    Steve Swinnen, Sílvia F. Henriques, Ranjan Shrestha, Ping-Wei Ho, Isabel Sá-Correia and Elke Nevoigt

    Microbial Cell Factories 2017 16:7

    Published on: 9 January 2017

  7. Research

    The ACEII recombinant Trichoderma reesei QM9414 strains with enhanced xylanase production and its applications in production of xylitol from tree barks

    ACEII transcription factor plays a significant role in regulating the expression of cellulase and hemicellulase encoding genes. Apart from ACEII, transcription factors such as XYR1, CRE1, HAP2/3/5 complex and ...

    Lili Xiong, Ayyappa Kumar Sista Kameshwar, Xi Chen, Zhiyun Guo, Canquan Mao, Sanfeng Chen and Wensheng Qin

    Microbial Cell Factories 2016 15:215

    Published on: 28 December 2016

  8. Research

    Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

    Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives.

    Jung-Hun Kim, Chonglong Wang, Hui-Jung Jang, Myeong-Seok Cha, Ju-Eon Park, Seon-Yeong Jo, Eui-Sung Choi and Seon-Won Kim

    Microbial Cell Factories 2016 15:214

    Published on: 23 December 2016

  9. Research

    Proteins adopt functionally active conformations after type III secretion

    Bacterial production of natively folded heterologous proteins by secretion to the extracellular space can improve protein production by simplifying purification and enabling continuous processing. In a typical...

    Kevin James Metcalf, James Lea Bevington, Sandy Lisette Rosales, Lisa Ann Burdette, Elias Valdivia and Danielle Tullman-Ercek

    Microbial Cell Factories 2016 15:213

    Published on: 23 December 2016

  10. Research

    Mapping the membrane proteome of anaerobic gut fungi identifies a wealth of carbohydrate binding proteins and transporters

    Engineered cell factories that convert biomass into value-added compounds are emerging as a timely alternative to petroleum-based industries. Although often overlooked, integral membrane proteins such as solut...

    Susanna Seppälä, Kevin V. Solomon, Sean P. Gilmore, John K. Henske and Michelle A. O’Malley

    Microbial Cell Factories 2016 15:212

    Published on: 20 December 2016

  11. Research

    Rhodococcus aetherivorans BCP1 as cell factory for the production of intracellular tellurium nanorods under aerobic conditions

    Tellurite (TeO3 2−) is recognized as a toxic oxyanion to living organisms. However, mainly anaerobic or facultative-anaerobic microorganisms are able to tolerate and convert TeO3 ...

    Alessandro Presentato, Elena Piacenza, Max Anikovskiy, Martina Cappelletti, Davide Zannoni and Raymond J. Turner

    Microbial Cell Factories 2016 15:204

    Published on: 15 December 2016

  12. Research

    Elimination of N-glycosylation by site mutation further prolongs the half-life of IFN-α/Fc fusion proteins expressed in Pichia pastoris

    Interferon (IFN)-α has been commonly used as an antiviral drug worldwide; however, its short half-life in circulation due to its low molecular weight and sensitivity to proteases impacts its efficacy and patie...

    Hao Jia, Yugang Guo, Xiaoping Song, Changsheng Shao, Jing Wu, Jiajia Ma, Mingyang Shi, Yuhui Miao, Rui Li, Dong Wang, Zhigang Tian and Weihua Xiao

    Microbial Cell Factories 2016 15:209

    Published on: 7 December 2016

  13. Research

    Microbial production of next-generation stevia sweeteners

    The glucosyltransferase UGT76G1 from Stevia rebaudiana is a chameleon enzyme in the targeted biosynthesis of the next-generation premium stevia sweeteners, rebaudioside D (Reb D) and rebaudioside M (Reb M). These...

    Kim Olsson, Simon Carlsen, Angelika Semmler, Ernesto Simón, Michael Dalgaard Mikkelsen and Birger Lindberg Møller

    Microbial Cell Factories 2016 15:207

    Published on: 7 December 2016

  14. Research

    Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum

    Technical bulk enzymes represent a huge market, and the extracellular production of such enzymes is favorable due to lowered cost for product recovery. Protein secretion can be achieved via general secretion (...

    Johannes Hemmerich, Peter Rohe, Britta Kleine, Sarah Jurischka, Wolfgang Wiechert, Roland Freudl and Marco Oldiges

    Microbial Cell Factories 2016 15:208

    Published on: 7 December 2016

  15. Research

    Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate

    Microtiter plates (MTP) are often applied as culture vessels in high-throughput screening programs. If online measuring techniques are available, MTPs can also be applied in the first steps of process developm...

    Tobias Ladner, Markus Held, David Flitsch, Mario Beckers and Jochen Büchs

    Microbial Cell Factories 2016 15:206

    Published on: 3 December 2016

  16. Review

    Current knowledge and perspectives of Paenibacillus: a review

    Isolated from a wide range of sources, the genus Paenibacillus comprises bacterial species relevant to humans, animals, plants, and the environment. Many Paenibacillus species can promote crop growth directly via...

    Elliot Nicholas Grady, Jacqueline MacDonald, Linda Liu, Alex Richman and Ze-Chun Yuan

    Microbial Cell Factories 2016 15:203

    Published on: 1 December 2016

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