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  1. Research

    Application of an E. coli signal sequence as a versatile inclusion body tag

    Heterologous protein production in Escherichia coli often suffers from bottlenecks such as proteolytic degradation, complex purification procedures and toxicity towards the expression host. Production of proteins...

    Wouter S. P. Jong, David Vikström, Diane Houben, H. Bart van den Berg van Saparoea, Jan-Willem de Gier and Joen Luirink

    Microbial Cell Factories 2017 16:50

    Published on: 21 March 2017

  2. Research

    Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

    Pichia pastoris is a widely used eukaryotic expression host for recombinant protein production. Adaptive laboratory evolution (ALE) has been applied in a wide range of studies in order...

    Josef W. Moser, Roland Prielhofer, Samuel M. Gerner, Alexandra B. Graf, Iain B. H. Wilson, Diethard Mattanovich and Martin Dragosits

    Microbial Cell Factories 2017 16:49

    Published on: 17 March 2017

  3. Research

    Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies

    Transcriptional reprogramming is a fundamental process of living cells in order to adapt to environmental and endogenous cues. In order to allow flexible and timely control over gene expression without the int...

    Emil D. Jensen, Raphael Ferreira, Tadas Jakočiūnas, Dushica Arsovska, Jie Zhang, Ling Ding, Justin D. Smith, Florian David, Jens Nielsen, Michael K. Jensen and Jay D. Keasling

    Microbial Cell Factories 2017 16:46

    Published on: 15 March 2017

  4. Research

    Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae

    Oxidized glutathione (GSSG) is the preferred form for industrial mass production of glutathione due to its high stability compared with reduced glutathione (GSH). In our previous study, over-expression of the ...

    Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y. Hara, Tomohisa Hasunuma and Akihiko Kondo

    Microbial Cell Factories 2017 16:44

    Published on: 15 March 2017

  5. Research

    Dynamic regulation of fatty acid pools for improved production of fatty alcohols in Saccharomyces cerevisiae

    In vivo production of fatty acid-derived chemicals in Saccharomyces cerevisiae requires strategies to increase the intracellular supply of either acyl-CoA or free fatty acids (FFAs), since their cytosolic concent...

    Paulo Gonçalves Teixeira, Raphael Ferreira, Yongjin J. Zhou, Verena Siewers and Jens Nielsen

    Microbial Cell Factories 2017 16:45

    Published on: 15 March 2017

  6. Research

    Functional differentiation of 3-ketosteroid Δ1-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4

    The Rhodococcus ruber strain Chol-4 genome contains at least three putative 3-ketosteroid Δ1-dehydrogenase ORFs (kstD1, kstD2 and kstD3) that code for flavoenzymes involved in the steroid ring degradation. The ai...

    Govinda Guevara, Laura Fernández de las Heras, Julián Perera and Juana María Navarro Llorens

    Microbial Cell Factories 2017 16:42

    Published on: 14 March 2017

  7. Research

    Overexpression of a C4-dicarboxylate transporter is the key for rerouting citric acid to C4-dicarboxylic acid production in Aspergillus carbonarius

    C4-dicarboxylic acids, including malic acid, fumaric acid and succinic acid, are valuable organic acids that can be produced and secreted by a number of microorganisms. Previous studies on organic acid productio....

    Lei Yang, Eleni Christakou, Jesper Vang, Mette Lübeck and Peter Stephensen Lübeck

    Microbial Cell Factories 2017 16:43

    Published on: 14 March 2017

  8. Research

    Constitutively-stressed yeast strains are high-yielding for recombinant Fps1: implications for the translational regulation of an aquaporin

    We previously selected four strains of Saccharomyces cerevisiae for their ability to produce the aquaporin Fps1 in sufficient yield for further study. Yields from the yeast strains spt3Δ, srb5Δ, gcn5Δ and yTHCBMS...

    Stephanie P. Cartwright, Richard A. J. Darby, Debasmita Sarkar, Nicklas Bonander, Stephane R. Gross, Mark P. Ashe and Roslyn M. Bill

    Microbial Cell Factories 2017 16:41

    Published on: 9 March 2017

  9. Review

    Trends in recombinant protein use in animal production

    Recombinant technologies have made possible the production of a broad catalogue of proteins of interest, including those used for animal production. The most widely studied proteins for the animal sector are t...

    Laia Gifre, Anna Arís, Àlex Bach and Elena Garcia-Fruitós

    Microbial Cell Factories 2017 16:40

    Published on: 4 March 2017

  10. Review

    Cellular factories for coenzyme Q10 production

    Coenzyme Q10 (CoQ10), a benzoquinone present in most organisms, plays an important role in the electron-transport chain, and its deficiency is associated with various neuropathies and muscular disorders. CoQ10 is...

    Sean Qiu En Lee, Tsu Soo Tan, Makoto Kawamukai and Ee Sin Chen

    Microbial Cell Factories 2017 16:39

    Published on: 2 March 2017

  11. Research

    Transcriptional activator Cat8 is involved in regulation of xylose alcoholic fermentation in the thermotolerant yeast Ogataea (Hansenula) polymorpha

    Efficient xylose alcoholic fermentation is one of the key to a successful lignocellulosic ethanol production. However, regulation of this process in the native xylose-fermenting yeasts is poorly understood. In...

    Justyna Ruchala, Olena O. Kurylenko, Nitnipa Soontorngun, Kostyantyn V. Dmytruk and Andriy A. Sibirny

    Microbial Cell Factories 2017 16:36

    Published on: 28 February 2017

  12. Research

    Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems

    Cellobiose dehydrogenase (CDH) is an extracellular enzyme produced by lignocellulolytic fungi. cdh gene expression is high in cellulose containing media, but relatively low CDH concentrations are found in the sup...

    Su Ma, Marita Preims, François Piumi, Lisa Kappel, Bernhard Seiboth, Eric Record, Daniel Kracher and Roland Ludwig

    Microbial Cell Factories 2017 16:37

    Published on: 28 February 2017

  13. Research

    Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1

    Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production o...

    Antonio Juarez, Juan A. Villa, Val F. Lanza, Beatriz Lázaro, Fernando de la Cruz, Héctor M. Alvarez and Gabriel Moncalián

    Microbial Cell Factories 2017 16:35

    Published on: 27 February 2017

  14. Research

    Ethylene production with engineered Synechocystis sp PCC 6803 strains

    Metabolic engineering and synthetic biology of cyanobacteria offer a promising sustainable alternative approach for fossil-based ethylene production, by using sunlight via oxygenic photosynthesis, to convert c...

    Vinod Puthan Veetil, S. Andreas Angermayr and Klaas J. Hellingwerf

    Microbial Cell Factories 2017 16:34

    Published on: 23 February 2017

  15. Research

    Artificially designed recombinant protein composed of multiple epitopes of foot-and-mouth disease virus as a vaccine candidate

    Concerns regarding the safety of inactivated foot-and-mouth disease (FMD) vaccine have been raised since it is produced from cultured live FMD virus (FMDV). To overcome this issue, recombinant protein has been...

    Ho-Bin Lee, Da-Chuan Piao, Jun-Yeong Lee, Jae-Yun Choi, Jin-Duck Bok, Chong-Su Cho, Sang-Kee Kang and Yun-Jaie Choi

    Microbial Cell Factories 2017 16:33

    Published on: 22 February 2017

  16. Research

    Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica

    The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and ...

    Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud and Christophe Leplat

    Microbial Cell Factories 2017 16:31

    Published on: 17 February 2017

  17. Research

    Thermus thermophilus as source of thermozymes for biotechnological applications: homologous expression and biochemical characterization of an α-galactosidase

    The genus Thermus, which has been considered for a long time as a fruitful source of biotechnological relevant enzymes, has emerged more recently as suitable host to overproduce thermozymes. Among these, α-galact...

    Martina Aulitto, Salvatore Fusco, Gabriella Fiorentino, Danila Limauro, Emilia Pedone, Simonetta Bartolucci and Patrizia Contursi

    Microbial Cell Factories 2017 16:28

    Published on: 13 February 2017

  18. Research

    A high-throughput expression screening platform to optimize the production of antimicrobial peptides

    Antimicrobial peptides (AMPs) are promising candidates for the development of novel antibiotics, but it is difficult to produce sufficient quantities for preclinical and clinical studies due to their toxicity ...

    Christine Schreiber, Hagen Müller, Oliver Birrenbach, Moritz Klein, Doreen Heerd, Tobias Weidner, Denise Salzig and Peter Czermak

    Microbial Cell Factories 2017 16:29

    Published on: 13 February 2017

  19. Research

    Secretion of biologically active pancreatitis-associated protein I (PAP) by genetically modified dairy Lactococcus lactis NZ9000 in the prevention of intestinal mucositis

    Mucositis is one of the most relevant gastrointestinal inflammatory conditions in humans, generated by the use of chemotherapy drugs, such as 5-fluoracil (5-FU). 5-FU-induced mucositis affects 80% of patients ...

    Rodrigo D. Carvalho, Natalia Breyner, Zelia Menezes-Garcia, Nubia M. Rodrigues, Luisa Lemos, Tatiane U. Maioli, Danielle da Gloria Souza, Denise Carmona, Ana M. C. de Faria, Philippe Langella, Jean-Marc Chatel, Luis G. Bermúdez-Humarán, Henrique C. P. Figueiredo, Vasco Azevedo and Marcela S. de Azevedo

    Microbial Cell Factories 2017 16:27

    Published on: 13 February 2017

  20. Research

    Current lipid extraction methods are significantly enhanced adding a water treatment step in Chlorella protothecoides

    Microalgae have the potential to rapidly accumulate lipids of high interest for the food, cosmetics, pharmaceutical and energy (e.g. biodiesel) industries. However, current lipid extraction methods show effic...

    Xiaojie Ren, Xinhe Zhao, François Turcotte, Jean-Sébastien Deschênes, Réjean Tremblay and Mario Jolicoeur

    Microbial Cell Factories 2017 16:26

    Published on: 11 February 2017

  21. Research

    Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis

    Poly-gamma-glutamic acid (γ-PGA) is a promising macromolecule with potential as a replacement for chemosynthetic polymers. γ-PGA can be produced by many microorganisms, including Bacillus species. Bacillus lichen...

    Wencheng Yu, Zhen Chen, Hong Ye, Peize Liu, Zhipeng Li, Yuanpeng Wang, Qingbiao Li, Shan Yan, Chuan-jian Zhong and Ning He

    Microbial Cell Factories 2017 16:22

    Published on: 8 February 2017

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