Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine
© Peifer et al.; licensee BioMed Central Ltd. 2012
Received: 31 August 2012
Accepted: 21 October 2012
Published: 24 October 2012
Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism. Further, IMP and GMP represent industrially relevant biotechnological products used as flavor enhancing additives in food industry. Therefore, this work aimed towards the accumulation of IMP applying targeted genetic engineering of Corynebacterium glutamicum.
Blocking of the degrading reactions towards AMP and GMP lead to a 45-fold increased intracellular IMP pool of 22 μmol gCDW-1. Deletion of the pgi gene encoding glucose 6-phosphate isomerase in combination with the deactivated AMP and GMP generating reactions, however, resulted in significantly decreased IMP pools (13 μmol gCDW-1). Targeted metabolite profiling of the purine biosynthetic pathway further revealed a metabolite shift towards the formation of the corresponding nucleobase hypoxanthine (102 μmol gCDW-1) derived from IMP degradation.
The purine biosynthetic pathway is strongly interconnected with various parts of the central metabolism and therefore tightly controlled. However, deleting degrading reactions from IMP to AMP and GMP significantly increased intracellular IMP levels. Due to the complexity of this pathway further degradation from IMP to the corresponding nucleobase drastically increased suggesting additional targets for future strain optimization.
KeywordsPurine accumulation Metabolic engineering Corynebacterium glutamicum Targeted metabolomics Metabolic flux analysis
The diverse class of purine intermediates comprises phosphorylated nucleotides, nucleosides and nucleobases, exhibiting multiple functions in the cellular system : for example, they serve as transmitters of the genetic information , as phosphate group donors, are involved in signal mediation  and ensure the energy supply of the living cell . Thus, these intermediates are involved in almost every aspect of cellular metabolism. Furthermore, some of these compounds possess secondary functions as flavor enhancing substances, i.e. IMP and GMP [5–7], and act as potential drugs in medical therapy, e.g. inosine and purine analogs [8–10]. Due to their immense pharmaceutical and biotechnological potential, these compounds have been successfully synthesized over the past decades via fermentative production employing Corynebacteria, especially Corynebacterium ammoniagenes[11–13].
De novo nucleotide synthesis begins with the activation of the pentose phosphate pathway (PPP) intermediate and direct precursor ribose 5-phosphate , which is further converted to IMP in a twelve-step biosynthetic process with concomitant consumption of 10 mol ATP. AMP and GMP are derived from IMP as depicted in Figure 1[1, 17]. The salvage pathway, however, converts extracellular nucleobases or degraded purine compounds into the corresponding nucleosides and nucleotides [11, 18], thereby preventing energetically expensive de novo synthesis of these compounds.
Since primary metabolism is designed and optimized to deliver and sustain a more or less consistent distribution of metabolites (described as network rigidity) – which can be used as cellular building blocks, redox equivalents, energy and co-factors – any kind of overproduction or accumulation basically targets the deregulation of the underlying metabolism. In the present study, a strategy for increased IMP accumulation was developed by focusing on modifying the general key factors for overproduction: (i) increasing the precursor availability; (ii) de-bottlenecking regulation-restricted branch points, and (iii) deactivating the depleting reactions. The goal of this study was to identify essential nodes of this pathway and to uncover impacts from central and purine metabolism. Applying genetic engineering and in-depth metabolome analysis of the purine pathway intermediates, information of the extremely well-regulated purine pathway should be gathered [1, 17, 20, 21]. However, the underlying mechanisms and the contributions of different branches are not well described yet. In addition, identification of individual regulation factors [22, 23] operating at different hierarchic levels  is challenging due to the comprehensive connectivity of the metabolic network. The interconnectivity of metabolome and fluxome therefore provides valuable insights into global dynamics [25, 26] resulting from single and multiple perturbations in the central and branch metabolism .
Metabolic snapshots of the purine pathway in wild type C. glutamicum
Concentrations of purine intermediates [μmol g CDW -1 ] determined for C. glutamicum wild type (ATCC 13032) and site-directed mutants a
Concentration [μmol gCDW-1]
ΔpurA ΔguaB2 purF K348Q Δpgi
8.13 ± 0.50
9.07 ± 0.52
10.24 ± 0.78
10.66 (17.93) ± 0.22 (1.18)
10.11 (27.79) ± 0.05 (2.03)
3.69 ± 0.54
3.57 ± 0.20
0.10 ± 0.01
0.09 ± 0.01
0.12 ± 0.02
0.13 ± 0.01
0.21 ± 0.01
0.05 ± 0.00
0.15 ± 0.03
2.63 ± 0.25
3.04 ± 0.11
3.36 ± 0.30
3.04 (5.58) ± 0.38 (0.26)
4.03 (8.32) ± 0.13 (0.53)
2.63 ± 0.16
3.65 ± 0.17
4.75 ± 0.27
9.47 (21.10) ± 0.12 (2.03)
3.92 (2.33) ± 0.19 (0.24)
0.65 ± 0.07
0.51 ± 0.03
0.04 ± 0.00
0.03 ± 0.00
0.03 ± 0.01
0.06 ± 0.01
0.10 ± 0.01
0.23 ± 0.02
3.27 ± 0.28
3.85 ± 0.17
5.08 ± 0.61
5.07 (10.27) ± 1.45 (0.62)
35.67 (9.38) ± 1.65 (0.64)
0.49 ± 0.06
0.42 ± 0.03
0.55 ± 0.05
30.72 (43.99) ± 0.48 (3.32)
6.39 (37.16) ± 0.21 (3.48)
21.91 ± 0.89
12.88 ± 0.74
0.13 ± 0.05
0.21 ± 0.04
0.38 ± 0.12
0.28 ± 0.06
0.85 ± 0.06
1.26 ± 0.08
2.53 ± 0.19
0.28 ± 0.03
0.29 ± 0.03
0.28 ± 0.03
11.87 (17.92) ± 1.00 (1.26)
3.55 (16.19) ± 0.04 (1.34)
101.53 ± 1.09
0.12 ± 0.01
0.22 ± 0.01
0.11 ± 0.00
0.17 ± 0.00
All compounds except xanthosine and XMP, which were below the detection limit, were quantified with a precision better than 12%. AMP and GMP, as central building constituents for DNA and RNA and co-substrates for various enzyme reactions [2, 3], showed the highest concentration ranges of 8.13 and 2.63 μmol gCDW-1, respectively. Other intermediates varied within a broader concentration range from 0.04 to 3.27 μmol gCDW-1. The lowest concentrations were detected for nucleosides reaching up to 0.04 μmol gCDW-1 for guanosine.
Several genetic perturbations aiming at various metabolic key nodes were performed to evaluate the effects and impacts of changing activities of different metabolic branches of the metabolism on the intracellular purine pool sizes.
Manipulating the main carbon flux distribution
Growth parameters for C. glutamicum ATCC 13032 and site-directed mutants
0.443 ± 0.004
0.095 ± 0.004
4.67 ± 0.09
0.376 ± 0.001
0.090 ± 0.002
4.18 ± 0.02
0.440 ± 0.008
0.091 ± 0.001
4.82 ± 0.06
0.455 ± 0.006
0.090 ± 0.001
5.05 ± 0.02
0.320 ± 0.007
0.073 ± 0.004
4.38 ± 0.03
0.317 ± 0.006
0.075 ± 0.001
4.24 ± 0.01
ΔpurA ΔguaB2 purF K348Q Δpgi
0.146 ± 0.003
0.076 ± 0.003
1.92 ± 0.02
Phenotypic analysis was further extended towards the determination of free intracellular purine pool sizes and a metabolic profile based on these analyses was generated (Table 1). Intracellular concentrations related to cell dry weight showed only minor changes compared to wild type C. glutamicum. GMP and AMP concentrations were slightly increased in C. glutamicum Δpgi, as were amounts of adenine, guanine and inosine. Adenosine and guanosine showed decreased concentrations. IMP and hypoxanthine were not affected significantly.
Targeted perturbations of the purine biosynthetic pathway
Further genetic manipulations aimed at the purine pathway itself, to estimate immediate effects emanating from this metabolic branch. The first target was the purF gene encoding the PRPP amidotransferase. This enzyme is generally regarded to act as key controller in the purine de novo synthesis, regulated by a feedback inhibition mainly caused by GMP and AMP [7, 33]. An experimentally identified amino acid substitution (K326Q) in the purF gene is reported to offset this feedback inhibition by AMP and GMP in E. coli and was incorporated into the corresponding position in the purF gene of C. glutamicum. In order to check for evolutionary conserved coding regions, a sequence alignment covering the complete amino acid sequence was performed . Therefore, six organisms exhibiting a broad phylogenetic distinction, including Escherichia coli, Corynebacterium glutamicum, Corynebacterium ammoniagenes, Bacillus subtilis, Salmonella typhimurium and Saccharomyces cerevisiae, were analyzed. A conserved region comprising at least ten amino acids was identified for all organisms including the identified location of the amino acid substitution K326Q in E. coli described by Zhou et al. . The corresponding substitution K348Q in C. glutamicum was inserted into the genomic DNA. Verification of the base exchange was carried out by sequencing.
The amino acid substituent C. glutamicum purF K348Q (purF K348Q ) showed balanced growth with stable biomass formation and growth rate (Table 2). Intracellular purine intermediate pool sizes remained almost unchanged for adenosine, guanosine and hypoxanthine (Table 1). The most striking changes were observed for intracellular inosine concentration which increased 2.9-fold. Compared to the wild type strain, the IMP concentration, however, showed only a minor change (10% increase). Intracellular concentrations of AMP and its corresponding nucleobase adenine increased by approximately 25% each. GMP and guanine pool sizes in C. glutamicum purF K348Q increased significantly by 80% and 55%, respectively, compared to the wild type, indicating a global shift towards the main intermediates GMP, AMP and inosine. Direct determination of the in vitro activity of PurF following the method of Matsui et al. , employing HPLC quantitation of substrate (glutamine) and product (glutamate) concentrations  over time in crude cell extracts, resulted in tremendous background activity, not permitting kinetic studies. In addition, heterologous expression using Escherichia coli as expression system resulted in enzyme inactivation, probably due to complex posttranslational events. Further studies will however focus on the determination of the in vitro activity.
In addition to the deregulation of PurF, further genetic manipulations aimed at IMP degradation, affecting the synthesis of IMP-derived GMP and AMP. The biosynthesis of both intermediates was thus shifted from de novo biosynthesis to the purine salvage pathway, employing the two enzymes adenosine-phosphoribosyltransferase (APRT) and hypoxanthine-guanine-phosphoribosyltransferase (HGPRT) catalyzing the synthesis of AMP and GMP/IMP via a condensing reaction of adenine and guanine/hypoxanthine with PRPP maintaining a sustained supply of the necessary precursors. These genetic manipulations, however, lead to auxotrophies for adenine and/or guanine [35, 37].
As initial strategy, the single gene deletions of the adenylosuccinate synthetase (purA) (catalyzing the conversion of IMP to AMP) and of the IMP-dehydrogenase (guaB2) (catalyzing the reaction of IMP to GMP) were analyzed. Due to the generated auxotrophies, the medium had to be supplied with adenine and guanine and resulting phenotypes based on intracellular concentrations of the corresponding purine pathway intermediates were analyzed (Table 1).
The metabolic shift from de novo purine biosynthesis to the salvage pathway did not show any significant changes in the growth rate of the purA deletion strain. The guaB2 deletion strain, however, showed a drastic response after redirecting GMP formation to the salvage pathway: biomass yield was reduced by 25% (0.073 g mol-1glucose) of the wild type yield and the specific growth rate even dropped by 30% of the wild type rate, reaching a stable rate of 0.32 h-1, thus indicating a restricted compensation efficiency of the salvage pathway concerning the GMP supply. Metabolic phenotyping revealed even more distinct alterations: most strikingly both deletion mutants were characterized by unbalanced intracellular pools sizes, exhibiting transient concentration changes which could not be related to biomass formation (Table 1). Degradation inefficiency of IMP towards the following nucleotides (GMP and AMP) led to maximal intracellular IMP concentrations of 44 μmol gCDW-1 (ΔpurA) and 37 μmol gCDW-1 (ΔguaB2) corresponding to a 90- and 80-fold increase compared to the wild type strain. Hypoxanthine concentrations were affected likewise.
The unbalanced metabolic state observed in C. glutamicum ΔpurA and C. glutamicum ΔguaB2, was further investigated in the double deletion mutant C. glutamicum ΔpurA ΔguaB2, exhibiting auxotrophies for adenine as well as for guanine. Specific growth parameters of the double deletion mutant, i.e. biomass yield (0.075 gCDW mmolglucose-1) and specific growth rate (0.317 h-1), were consistent with those obtained for C. glutamicum ΔguaB2, indicating a mutual growth-related effect. However, the metabolic fingerprint for C. glutamicum ΔpurA ΔguaB2 exhibited remarkable differences (Table 1): the unbalanced metabolic behavior observed in C. glutamicum ΔpurA and C. glutamicum ΔguaB2 was completely reestablished resulting in constant intracellular purine intermediate concentrations for all substances quantified. The double deletion mutant showed a decreased intracellular IMP pool of 21.9 μmol gCDW-1 compared to the single gene deletion mutants which corresponds to a 44-fold increase compared to the wild type strain. The IMP reduction was accompanied by a significant increase of the inosine concentration (9.7-fold compared to the wild type strain) and especially hypoxanthine (290-fold compared to the wild type strain), indicating a metabolic shift from main purine intermediates; i.e., the nucleotides, to the corresponding degradation products (nucleobases).
Extended metabolic manipulations – combination of central pathway and branch pathway modifications
Finally, the combined influence of the genetic modifications described above was investigated. The rationally designed mutant C. glutamicum ΔpurA ΔguaB2 purF K348Q Δpgi is characterized by the amino acid substitution (purF K348Q), supposed to affect the regulatory key enzyme for the de novo synthesis. In addition, the mutant exhibits gene deletions for the initiating reaction of glycolysis (pgi) and for the converting reactions leading from IMP to the successive purine nucleotides AMP (purA) and GMP (guaB2).
The biomass yield of 0.076 gCDW mmolglucose-1 for C. glutamicum ΔpurA ΔguaB2 purF K348Q Δpgi was consistent with those obtained for C. glutamicum ΔpurA ΔguaB2 and C. glutamicum ΔguaB2. However, the specific growth rate was reduced to 0.146 h-1. In contrast to the two single gene deletion mutants (ΔpurA and ΔguaB2), the previously observed IMP decline observed in the double deletion mutant C. glutamicum ΔpurA ΔguaB2 was even more pronounced in the mutant containing the four genetic modifications (Table 1). In conjunction with these findings, an increased IMP degradation towards the corresponding nucleoside inosine and nucleobase hypoxanthine was observed.
Quantification of intracellular carbon flux distributions
Alterations of in vivo carbon flux distributions were investigated in order to assess the impact on the purine intermediate profile. The network contained reactions of glycolysis, TCA cycle, anaplerosis and PPP. Estimation of intracellular flux distributions was carried out based on measurable extracellular rates, i.e. glucose consumption, biomass and by-product formation as well as the mass isotopomer data obtained from 13C enriched cellular protein. Initial values were statistically varied and multiple parameter estimations were performed to guarantee finding of the global minimum solution.
Deletion of the pgi gene strongly affected the carbon flux distribution and caused a complete carbon redirection towards the PPP. The reflux of ribose 5-phosphate via the non-oxidative part of the PPP entering glycolysis was almost tripled. Activity of the TCA cycle was only slightly affected, showing a small reduction from 47.3% in the wild type strain to 45.7% in the pgi deletion mutant.
In contrast, the double deletion mutant C. glutamicum ΔpurA ΔguaB2, possessing an intact pgi gene, exhibited the lowest flux through the oxidative part of the PPP (29.3%), which almost exclusively accounted for biomass formation. The relative reflux into glycolysis was less than 10%. TCA cycle activity was increased to 88%. The strain C. glutamicum ΔpurA ΔguaB2 purF K348Q Δpgi showed a carbon flux redirection from glycolysis towards the PPP (98.0%) caused by the pgi gene deletion.
Perturbation-caused alterations of the NADH metabolism
The consequences of the unequal NADH/FADH2 supply are discussed in more detail in “Cluster analysis” ( see section Discussion).
Rerouting metabolic fluxes – contributions of individual pathway branches with respect to purine accumulation
Targeted perturbations in the purine biosynthetic pathway
Imbalances in free intracellular purine pools are reported to be mutagenic and genotoxic [20, 21]. Thus a prerequisite for balanced cellular growth is to maintain the intracellular pool sizes, which resulted in efficient regulatory mechanisms [1, 16, 17, 33, 35, 38]. This pool stability is on the one hand sustained by an interaction of de novo purine synthesis and the salvage pathway, ensuring a constant supply of purine nucleotides required for cellular growth . On the other hand, increasing concentrations perturbing the system can easily be broken down via the present degrading and converting reactions.
In case of the purA gene deletion mutant (C. glutamicum ΔpurA), extracellular supplementation with adenine completely restored cellular growth, resulting in comparable specific growth rates of the wild type (0.443 h-1) and the C. glutamicum ΔpurA strain (0.455 h-1), indicating a thorough compensatory effect by the salvage pathway.
On the contrary, extracellular guanine complementation was not sufficient to restore wild type growth behavior in the guaB2 gene deletion mutant (C. glutamicum ΔguaB2): specific growth rate as well as biomass yield were reduced by 25%, each compared to the wild type strain (Table 2). This can either be explained by insufficient uptake or converting systems for guanine. The latter is catalyzed by the salvage pathway. The observed phenotypic consequences reemerged in every genotype comprising this genetic perturbation (C. glutamicum ΔguaB2 and C. glutamicum ΔpurA ΔguaB2), no matter if single or multiple genes were deleted. The attribution of this fundamental salvage branch restriction regarding uptake or conversion could not be answered definitely in this study. Decreasing intracellular guanine concentrations in C. glutamicum ΔguaB2, however indicate an uptake insufficiency (and not a conversion insufficiency!). Decreasing metabolite concentrations are caused by increased consuming reactions (in this case by the salvage pathway).
In contrast, the purA gene deletion mutant C. glutamicum ΔpurA exhibited increasing adenine concentrations, supporting a sufficient adenine uptake, even exceeding converting reactions by the salvage pathway. This theory is additionally supported by the reduced guanine uptake (125 μmol gCDW-1) compared to the adenine uptake (243 μmol gCDW-1), indicating basic differences for both intermediates.
Regarding intracellular pool sizes, the single gene deletion mutants C. glutamicum ΔpurA and C. glutamicum ΔguaB2 were found to exhibit the most promising rises in intracellular IMP concentration with maximal IMP pool sizes of 44.0 and 37.2 μmol gCDW-1, respectively. These associated transient concentration changes showed no relation to biomass formation and resulted in unstable and thus unrepresentative results. This phenomenon may at least partially be associated with direct changes in the GMP and AMP pool sizes (Table 1). These two compounds are reported to act as the most stringent regulators for the purine biosynthetic pathway by a feedback-mediated inhibition of the PurF enzyme . With both intermediates operating as regulators, it is not surprising that the individual gene deletions cause spreading influences on the whole branching pathway.
Targeted perturbation in the central carbon metabolism
Increasing the precursor supply via the oxidative branch of the PPP seemed to be a promising route to boost intracellular purine levels. This strategy has already been successfully applied for tryptophan production in E. coli and lysine production in C. glutamicum[40, 41], illustrating positive correlations between enhanced productivity and increased PPP activities .
Thus, the genetic modification (deletion of the pgi gene) was implemented into the genome. The resulting affects on the purine intermediate pool sizes (Table 1) however, were rather limited compared to those observed for purA and guaB2 gene deletion strains, indicating the strong regulation and rigidity of this pathway.
Effects of combined perturbations on intracellular purine pool sizes
Strains used for industrial manufacturing of purine intermediates are usually characterized by an adenine auxotrophy [35, 37, 43–45]. This perturbation seems to be fundamental, revealing the importance of an IMP conversion block for the accumulation of this intermediate metabolite . The extracellular supplementation with adenine, however, is linked to a repression of transcription initiation  of the genes of the pur operon  via the depletion of PRPP as described for B. subtilis. In order to counterbalance this phenomenon, the carbon flux was rerouted by introducing the pgi gene deletion, aiming at an increased PRPP supply (Figure 2).
The carbon flux was successfully rerouted. Intracellular purine levels though further shifted from nucleotides towards their degradation products, mainly inosine and hypoxanthine (Table 1).
The disruption of critical nodes in the purine pathway itself, i.e. the conversion of IMP to AMP and GMP, provided the required fundamental basis for far-reaching effects when combined with additional modifications as the pgi gene deletion.
Solely based on the metabolome data, no clear link between the additional genetic manipulation of the pgi deletion and the increased degradation shift could be concluded. Therefore, the research was extended by an in-depth fluxome analysis to capture integrative effects of fluxome and metabolome.
Connectivity of targeted metabolome and fluxome analysis
In the context of purine accumulation, alterations in the PPP-TCA cycle ratio were of special interest, as their relative activities and especially the flux partitioning into these two main branches, mainly dictate the XADH (NADH+FADH2) supply and thus ATP formation in the respiratory chain . The exclusive metabolization of glucose 6-phosphate via the PPP caused a major carbon flux redistribution in C. glutamicum ΔpurA ΔguaB2 purF K348Q Δpgi, exhibiting an adverse effect on XADH formation compared to C. glutamicum ΔpurA ΔguaB2 (Figure 4) and initiating a shift in the metabolic profile towards inosine and especially hypoxanthine formation. Since most XADH is generated in the TCA cycle, high PPP activities inevitable lead to an increased CO2 production via a decarboxylation reaction in the oxidative part of the PPP and thus to a reduced carbon availability for other metabolic branches, as seen for C. glutamicum ΔpurA ΔguaB2 purF K348Q Δpgi (Figure 2). Within the scope of the tightly regulated purine biosynthesis, Noguchi et al.  presumed a connection between a declining ATP supply and an increasing degrading efficiency within this pathway. The results obtained in this study are consistent with those obtained previously .
This suggests further metabolic engineering targets to increase intracellular IMP levels focusing on IMP degrading reactions.
In the present study, we performed an in-depth metabolic profiling of the purine biosynthetic pathway in C. glutamicum. Site-directed perturbations on the genetic level with subsequent studies of growth behavior and targeted metabolome analysis revealed a tight regulation of the underlying pathway with strong additional effects on the carbon flux distributions in the main metabolism as determined for C. glutamicum ΔpurA ΔguaB2 and C. glutamicum ΔpurA ΔguaB2 purF K348Q Δpgi.
Our results underline the importance of combined analyses employing different hierarchical levels such as fluxome and metabolome to reveal trends which indicate active regulation mechanism. The regulatory processes maintaining balanced intracellular concentrations have not been fully discovered yet and necessitate further investigation. Complex future studies will thus focus on the redox and energy metabolism, which were beyond the scope of the present analyses. In addition, it might be interesting to further analyze the metabolic instabilities – detected for C. glutamicum ΔpurA and C. glutamicum ΔguaB2. These instabilities were caused by the alteration of the complex regulatory structure of this pathway branch, opening up a way for in-depth surveys of this central and essential pathway in prokaryotes as well as in eukaryotes.
In order to achieve an understanding of the global cellular response, individual parts of the underlying network, i.e. precursor and cofactor supply, energy and redox metabolism, should not be assumed as independent, individual but interconnected units, linked by various key metabolites such as ATP or NAD(P)H.
Organisms and plasmids
Genetically modified organisms constructed in the present study: Mutant strains of C. glutamicum based on the wild type strain (ATCC 13032) were constructed by site-directed mutagenesis
C. glutamicum ATCC 13032
American Type Culture Collection
C. glutamicum Δpgi
deletion of the pgi gene (NCgl0817)
C. glutamicum ΔpurA
deletion of the purA gene (NCgl2669)
C. glutamicum ΔguaB2
deletion of the guaB2 gene (NCgl0578)
C. glutamicum purF K348Q
Nucleotide exchange K348Q in the purF gene (NCgl2495)
C. glutamicum ΔpurA ΔguaB2
double deletion of purA and guaB2 genes
C. glutamicum ΔpurA ΔguaB2 purF 348 Δpgi
deletion of purA, guaB2 and pgi genes and allelic amino acid substitution K348Q in the purF gene
E. coli DH5α
F−endA1, hsdR17(rk−mk+) supE44, thi-1λ− recAI gyrA96 relA1 Φ80ΔlacAm15
E. coli NM522pTC
supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (r K−m K+) [F′ proAB lacI qZΔM15]
Mutant genotypes and corresponding site-specific primer sequences (F, forward; R, reverse) used for verification a
5′- GATCACGCGTATCCCTTCTCCGGCATC- 3′
5′- GATCTCTAGATCCAGCGACACGAATAATC- 3′
5′- GATCTCTAGAATGGATCGGATGTGACG- 3′
5′- GATCACGCGTCAATCGGTCAACCTGGT- 3′
5′- GATCACGCGTGAGTTTCTACCGGAGGA- 3′
5′- GATCTCTAGATCAGACTGGAAGTAACG- 3′
purF K348Q - F
5′- GATCACGCGTGATTGCGGACTGGTTAC- 3′
purF K348Q - R
5′- GATCTCTAGACTCCTGCTGCTGCGTATG- 3′
5′- GGCCAAGGCATGGTCCAG AACGCCTACGTTGGC- 3′
5′- GCCAACGTAGGCGTTCTG GACCATGCCTTGGCC- 3′
DNA preparation and recombinant DNA modifications
Oligonucleotide synthesis was performed by Sigma-Aldrich (Steinheim, Germany) and DNA sequencing was carried out by GATC (Konstanz, Germany). Plasmid construction and DNA purification were performed using standard techniques. Preparation of genomic DNA from C. glutamicum was carried out with the MasterPureTM Gram Positive DNA Purification Kit (Biozym Scientific GmbH, Germany). PCR reactions were performed in a TGradient-Cycler (Whatman–BiometraR) with Phusion polymerase (Biozym) for insert construction and JumpStart REDTaq Ready Mix (Sigma-Aldrich) for strain verification. PCR products were purified using NucleoSpin® Extract II Kit (Macherey-Nagel, Düren, Germany) and plasmids using HiYield® Plasmid Mini Kit (SLG, Gauting, Germany). Ligations were performed with Fast-LinkTM DNA Ligation Kit (Epicentre Biotechnologies, Madison, WI, USA). FastDigest® restriction enzymes were obtained from Fermentas (St. Leon-Rot, Germany) as well as 10 mM dNTP Mix and 1-kb DNA O’GeneRulerTM.
Precultures used for transformation of C. glutamicum, were grown on BHI++ medium (10 mL in 100 mL baffled shake flasks) and incubated for 12 h at 30°C. The main culture in BHI++ medium (80 mL in 1000 mL baffled shake flasks) was inoculated from exponentially growing precultures to an optical density OD660nm of 0.2. 400 mg isonicotinic acid hydrazide, 2.5 g glycine and 0.1 mL Tween 80 were dissolved in 20 mL H2O, sterilized by filtration and added to the main culture at an OD660nm of 0.6. Cells were further incubated until an OD660nm of 0.8 was reached and harvested by centrifugation (7 min, 4000 g, 4°C, Laborfuge 400R, Heraeus, Hanau, Germany). Cells were washed three times with pre-cooled wash buffer (10% glycerine, 8 mM tris hydrochloride, pH 7.4) and once with 10% glycerine. After the second resuspension in 6 mL 10% glycerine, 200 μL cells were subsequently used for transformation. Electroporation was performed using a BioRad Gene Pulser II (Hercules, California, USA) with the following parameters: 25 μF, 600 Ω and 2.5 kV. Cells were transferred into a 2 mL Eppendorf tube, mixed with 1 mL BHIS medium, followed by a heat shock for 5 min at 46°C. Regeneration was performed for 90 min at 30°C to allow recovery of the cells, which were then spread on BHISKan agar plates and incubated for 4 days.
Preparation of heat shock competent E. coli cells was performed as described by Inoue et al. . Transformation of E. coli DH5α and NM522pTC with the integrative pClik-derivates was performed by heat shock . Briefly, cells were incubated on ice for 30 min, heated at 45°C for 45 s and again cooled on ice for 2 min. Regeneration was performed for 1 h at 37°C after 900 μL SOC medium were added.
Targeted mutant generation
Site-directed mutations were introduced using the integrative vector pClik, which lacks an ori for C. glutamicum. Selection on KanR as resistance marker yielded transformed cells with genome-integrated plasmid DNA. The insertion of the plasmids thereby results from a single crossover recombinant event. The second homologous recombination event, generated by the sacB positive selection system , causes the deletion of the sacB gene and therefore allows growth on corresponding selective agar plates. Due to the sacB associated additional deletion of the native or the modified gene sequence, transformants either contain the wild type gene sequence or exhibit the targeted mutation. KanR-nonresistant and sucrose-insensitive clones were further verified by allelic-specific PCR for the presence of the targeted gene sequence. The corresponding gene sequence for a nucleotide exchange, i.e. purF K348Q , was first deleted and then replaced.
Yeats extract, beef extract, polypeptone, casamino acids, and brain heart infusion (BHI) were purchased from Difco Laboratories (Detroit, USA). 99% enriched glucose, i.e. [1-13C] and [1,2-13C2], were obtained from Cambridge Isotope Laboratories (Andover, MA., USA). All other chemicals and reagents of analytical grade were purchased from Sigma-Aldrich, Merck (Darmstadt, Germany), Fluka (Buchs, Switzerland) or Roth (Karlsruhe, Germany).
The first precultures were grown on complex media (pH 6.8) containing per liter: 10.0 g glucose, 2.5 g NaCl, 2.0 g urea, 5.0 g yeast extract, 5.0 g beef extract, 5.0 g polypeptone and 20.0 g casamino acids. For agar plates, the complex medium was supplemented with 20.0 g L-1 agar. Second precultures and main cultures were grown on minimal medium (pH 7.2) containing per liter: 15.0 g glucose, 4.0 g KH2PO4, 16.0 g Na2HPO4, 500 mg MgCl2 · 6H2O, 300 mg 3,4-dihydroxibenzoic acid, 100 mg CaCl2 · 2H2O, 100 μg cyanocobalamin, 750 μg thiamine, 4 μg pyridoxal phosphate, 100 μg biotin, 400 μg calcium pantothenate, 2 μg folic acid, 400 μg nicotinic acid, 200 μg 4-aminobenzoic acid, 400 μg pyridoxine · HCl, 2 mg inositol, 10 mg FeCl2 · 4H2O, 1 mg ZnCl2, 100 μg CuCl2, 20 μg NiCl2 · 6H2O, 20 μg Na2MoO4 · 2H2O, 500 μg boric acid, 100 μg KI, 100 μg CoCl2 · 6H2O and 10 mg MnCl2 · 4H2O. For tracer studies, naturally labeled glucose was replaced by 99% 13C enriched [1-13C] or [1,2-13C] glucose. Mutants of C. glutamicum exhibiting deletions of adenylosuccinate synthetase (purA) or IMP dehydrogenase (guaB2) were grown on minimal media additionally supplemented with 200 mg L-1 adenine sulfate or 80 mg L-1 guanine hydrochloride, due to the introduced auxotrophies.
Media used for transformation experiments of C. glutamicum contained per liter: 37 g BHI, 26.4 g (NH4)2SO4 and 40 g glucose for BHI++ and 37 g BHI and 46 g sorbitol for BHIS medium.
Cultivations were carried out in baffled shake flasks on an orbital shaker (Multitron 2, Infors AG, Bottmingen, Switzerland) at 230 rpm and 30°C. First precultures were grown on complex medium (25 mL medium in 250 mL baffled flask) for 8 h and used for inoculation of the second precultures (25 mL medium in 250 mL baffled flask). Second precultures and main cultures were performed in minimal medium. Main cultivations were performed in duplicates in minimal medium using 500 mL baffled shake flasks with 50 mL medium. For tracer experiments, which were conducted under the same conditions as main cultivations, naturally labeled glucose was replaced by [1-13C] glucose or [1,2-13C2] glucose for analysis of glucose 6-phosphate isomerase (pgi) deletion mutants and the volume was adjusted to 5 mL in 50 mL baffled shake flasks.
Substrate and product analysis
Biomass concentration was determined by measuring the optical density of the culture at 660 nm (OD660nm) using a photometer (Marsha Pharmacia Biotech, Freiburg, Germany) which was gravimetrically correlated with the cell dry weight The correlation factor was determined as 0.414 gCDW OD660nm-1. Extracellular substrates and secreted products were analyzed after 3 min centrifugation at 16,000 g. Quantification of glucose, α-ketoglutarate and trehalose was performed in diluted supernatants by HPLC (Kontron Instruments, Neufahrn, Germany). Separation was performed using an Aminex HPX-87H Bio-Rad column (300 x 7.8 mm; Hercules, USA), at 55°C with a constant flow rate of 0.8 mL min-1 (7 mM H2SO4 as mobile phase). Refraction index (RI) detection was used for quantification of sugars, and UV detection at 218 nm for organic acids and at 254 nm for quantification of supplemented adenine and guanine. Determination of amino acids was performed as described previously . Extracellular concentrations of adenine and guanine were determined by HPLC (Agilent 1290 Infinity; Agilent Technologies, Waldbronn, Germany) using a Phenomenex (Aschaffenburg, Germany) reversed-phase C18-column (Gemini 5u 110Å, 150 x 4.6 mm). Separations were carried out at 10°C with a constant flow of 0.8 mL min-1. Gradient elution was performed using 5 mM ammonium formate solution at pH 5.8 (eluent A) and acetonitrile:methanol:water mixture (45:45:10, eluent B) applying the following gradient: an initial isocratic step with 0% B (3.5 min) was followed by an increase to 30% within 10 min and to 100% within 1 min. The flushing step at 100% B was performed for 5 min. The column was reconditioned at 0% B for another 5 min.
Analysis of intracellular metabolites
Extraction and quantification of intracellular purine intermediates, i.e. nucleotides, nucleosides and nucleobases, by LC-ESI-MS/MS was performed as described previously .
Mass spectrometric 13C-labeling analysis
The mass isotopomer distributions of amino acids derived from cellular protein and of trehalose secreted during cultivation were determined by mass spectrometry–gas chromatography. Sample preparation and instrument settings were performed as described previously [41, 51, 52]. In order to ensure isotopic steady state, labeling patterns of amino acids and trehalose were determined for two biomass concentrations during mid-exponential growth. Metabolic (pseudo-) steady state was assessed by constant yields, uptake and secretion rates and constant intracellular pool sizes of purine intermediates.
Calculation of the in vivo metabolic fluxes of the C. glutamicum strains was carried out by fitting simulated mass isotopomer distributions to experimentally determined labeling patterns of amino acids derived from cellular protein and trehalose secreted during cultivation. The calculations were based on the approach of Yang et al.  implemented in Matlab (version 2008b, Mathworks Inc., Nattick, USA) using metabolite and isotopomer balancing. Anabolic demands for biomass synthesis and extracellular production rates ware additionally considered  and implemented in the metabolic network. Media supplementation with adenine and guanine – required by strains comprising adenylosuccinate synthetase and/or IMP dehydrogenase deletions – led to reduced anabolic demands for DNA and RNA syntheses. The corresponding reduced anabolic demand for 3-phosphoglycerate was considered. In addition, increased ribose 5-phosphate requirements due to increased intracellular pool sizes of IMP, determined for C. glutamicum ΔpurA ΔguaB2 purF K348Q Δpgi and C. glutamicum ΔpurA ΔguaB2, were considered likewise.
Measured GC-MS data were corrected for naturally occurring isotopes in the corresponding amino acid fragments and derivatization reagent respectively [55, 56]. A Monte Carlo approach with 100 independent runs was employed for the statistical analysis .
Mean concentration values for purine intermediates were calculated from four biological replicates for each strain for two biomass concentrations and corrected using 13C-labeled cell extract as internal standard as described previously . The metabolomics-based phenotype profiling was extended by principal component analysis (PCA) applying pareto scaling of the data. Data sets were compressed by reduction of dimension numbers and the metabolomic patterns were highlighted. PCA was performed using Matlab R2007b (The Math Works, Inc., Boston, USA) software.
DAV acknowledges research support by the Alfried Krupp von Bohlen und Halbach-Stiftung.
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