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Table 1 Concentrations of purine intermediates [μmol g CDW -1 ] determined for C. glutamicum wild type (ATCC 13032) and site-directed mutants a

From: Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine

Compound

Concentration [μmol gCDW-1]

 

ATCC 13032

Δpgi

purF K348Q

ΔpurA c

ΔguaB2 c

ΔpurA ΔguaB2

ΔpurA ΔguaB2 purF K348Q Δpgi

AMP

8.13 ± 0.50

9.07 ± 0.52

10.24 ± 0.78

10.66 (17.93) ± 0.22 (1.18)

10.11 (27.79) ± 0.05 (2.03)

3.69 ± 0.54

3.57 ± 0.20

adenosine

0.10 ± 0.01

0.09 ± 0.01

0.12 ± 0.02

0.13 ± 0.01

0.21 ± 0.01

0.05 ± 0.00

0.15 ± 0.03

adenine

2.63 ± 0.25

3.04 ± 0.11

3.36 ± 0.30

3.04 (5.58) ± 0.38 (0.26)

4.03 (8.32) ± 0.13 (0.53)

b

b

GMP

2.63 ± 0.16

3.65 ± 0.17

4.75 ± 0.27

9.47 (21.10) ± 0.12 (2.03)

3.92 (2.33) ± 0.19 (0.24)

0.65 ± 0.07

0.51 ± 0.03

guanosine

0.04 ± 0.00

b

0.03 ± 0.00

0.03 ± 0.01

0.06 ± 0.01

0.10 ± 0.01

0.23 ± 0.02

guanine

3.27 ± 0.28

3.85 ± 0.17

5.08 ± 0.61

5.07 (10.27) ± 1.45 (0.62)

35.67 (9.38) ± 1.65 (0.64)

b

b

IMP

0.49 ± 0.06

0.42 ± 0.03

0.55 ± 0.05

30.72 (43.99) ± 0.48 (3.32)

6.39 (37.16) ± 0.21 (3.48)

21.91 ± 0.89

12.88 ± 0.74

inosine

0.13 ± 0.05

0.21 ± 0.04

0.38 ± 0.12

0.28 ± 0.06

0.85 ± 0.06

1.26 ± 0.08

2.53 ± 0.19

hypoxanthine

0.28 ± 0.03

0.29 ± 0.03

0.28 ± 0.03

11.87 (17.92) ± 1.00 (1.26)

3.55 (16.19) ± 0.04 (1.34)

80.65± 3.19

101.53 ± 1.09

XMP

b

b

b

b

n. d.

n. d.

n. d.

xanthosine

b

b

b

b

n. d.

n. d.

n. d.

xanthine

0.12 ± 0.01

0.22 ± 0.01

0.11 ± 0.00

0.17 ± 0.00

n. d.

n. d.

n. d.

  1. a Concentrations and corresponding standard deviations were determined using 4 biological replicates for each compound and standardized to the cell dry weight.
  2. b Below detection limit.
  3. c Purine intermediate concentrations determined for C. glutamicum ΔpurA and C. glutamicum ΔguaB2 represent maximum values obtained for two biomass concentrations for each strain: numbers on top correspond to the first biomass concentration (1.3 g L-1 for C. glutamicum ΔpurA and 1.1 g L-1 for C. glutamicum ΔguaB2) and numbers in brackets for second biomass concentration (1.9 g L-1 for C. glutamicum ΔpurA and 1.8 g L-1 for C. glutamicum ΔguaB2).
  4. n. d. not detected.