Table 2 Oligonucleotides used in this study
Oligonucleotides | Sequence |
|---|---|
Upstream of doeA F/R | tgacatgattacgccaagcttatgaaagtctttgaacaatgggaa/ccctccttgcaaggcctccaaagcatccg |
Downstream of doeA F/R | Tggaggccttgcaaggagggttactcaatggc/aaaacgacggccagtgaattcttaacccagccaaacctgctgc |
Integration to upstream of doeA F/R | atgcttccggctcgtatgtt / tccggatcgctgattacgac |
Integration to downstream of doeA F/R | gcgtcgaaggcgataaaacc / taagcccactgcaagctacc |
Confirmation of doeA deletion F/R | gcttcgaacgcggactacta / cgctgttgaggccgtaagtt |
Upstream of doeD F/R | tgacatgattacgccaagcttatggccatacagtgggatcagc/ gttcaatcatgaatactctccttacggttgacagg |
Downstream of doeD F/R | gagagtattcatgattgaacgcgacgacatg/ aaaacgacggccagtgaattcctaagccccgtattcgggt |
Integration to upstream of doeD F/R | cagtgagcgcaacgcaatta / cgcctgcgaagaagtcgata |
Integration to downstream of doeD F/R | gccttggcgaaaaacatcgt / ggacaggtcggtcaatcgtt |
Confirmation of doeD deletion F/R | cgattgagagatgtcggggg / cggtatcgggataaggtcgc |
Upstream of ectB F/R | tgacatgattacgccaagctttcaggacgcgaggcatattgc/ gagaattagaagcccgctgagccaaccag |
Downstream of ectB F/R | tcagcgggcttctaattctctcctgagcaagatgg/aaaacgacggccagtgaattcttaaaccggaaaatcaaacgc |
Integration to upstream of ectB F/R | ttgccgtcaggtgaaacgat / catgggctctgtttgagggg |
Integration to downstream of ectB F/R | ccgggtgattgtgaccgtaa / taagcccactgcaagctacc |
Confirmation of ectB deletion F/R | atgcatgcgaaaatcggcac / ggcagtgctgtgatgtttga |
RT-PCR for 16s rRNA gene F/R | tcccgggccttgtacacacc / gtggtaagcgccctcccgaa |
RT-PCR for doeA gene F/R | ggcgtcgaaggcgataaaac/ cccgtattcgggtttcacca |
RT-PCR for doeB gene F/R RT-PCR for doeD gene F/R | ctcacgaattcacacgcgac / tgcatcaatgtccggcgata gccttggcgaaaaacatcgt / caaagcatccgctaagacgc |