Table 1 Strains and plasmids used in this work
Strains and plasmids | Description | Sources | |
|---|---|---|---|
Escherichia coli | |||
DH10β | Used as a host for plasmid construction | Invitrogen | |
S17-1 | Donor in conjugative plasmid transfer | Â | |
Cupriavidus necator | |||
H16 | Wild type (ATCC 17,699, KCTC 22,469) | KCTC | |
ΔphaCAB | H16 derivative; ΔphaCAB | This study | |
PHB-4 | H16 derivative; phaC mutation (DSM-541) | KACC | |
Plasmids | |||
pBBR1-MCS2 | Broad host range plasmid; pPlac, Kmr, lacZ | Addgene | |
pBAD | pBBR1-MCS2 derivative with araBAD promoter and araC; Kmr | This study | |
pBAD-STS-TAL-4CL | pBAD derivative with VvSTS, FjTAL and At4CL | This study | |
pBAD-STS-TAL-4CL-ACC | pBAD derivative with VvSTS, FjTAL, At4CL and CgACC | This study | |
pBAD-STS-TAL-4CL-ACC-STS | pBAD derivative with 2 copies of VvSTS, FjTAL, At4CL and CgACC | This study | |
pJQ200mp18 | Suicide vector; sacB, oriV, oriT, traJ, Gmr | Addgene | |
pJQ200mp18Km | Derivative of pJQ200mp18, Kmr | This study | |
pJQ200mp18Km-phaCAB | pJQ200mp18Km carrying deletion cassette for phaCAB | This study | |