Fig. 4

Coumarate catabolic pathways in C. necator H16 causes the loss of p-coumaroyl CoA, an important precursor for resveratrol synthesis. (A) Cell growth of C. necator H16 strain on various aromatic compounds as the sole carbon source. PCA: p-coumaric acid, 4-HBA: 4-hydroxybenzoic acid. The initial optical density at 600 nm (OD₆₀₀) was 0.2 at 600 nm and the initial p-coumaric acid and 4-hydroxybenzoic acid concentrations were 3 and 5 mM, respectively. (B) Metabolic engineering of C. necator H16 for re-directing malonyl-CoA flux towards resveratrol synthesis by addition of _VvSTS