Fig. 4From: Combinatorial metabolic engineering of Bacillus subtilis enables the efficient biosynthesis of isoquercitrin from quercetinReducing the deglycosylation of isoquercitrin and enhancing the glycosylation of quercetin to improve glycosides accumulation. (A) The deglycosylation of isoquercitrin and the glycosylation of quercetin; (B) The biomass of glucosidases deletion in B. subtilis recombinants BSCG (initial host), BS221G (control), BS300G (ΔbglA), BS301G (ΔbglH), BS302G (ΔbglC), BS303G (ΔbglAΔbglC), BS304G (ΔbglCΔbglH), BS305G (ΔbglAΔbglH), BS306G (ΔbglAΔbglCΔbglH); (C) Changes of isoquercitrin titer by deleting glucosidases; (D) The concentrations of intracellular UDP-Glc in glucosidase disruption strains; (E) Changes of glucosidases activity by glucosidases disruptionBack to article page