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Table 1 Clones in liquid small-scale induction. Descriptions of all clones included in the small-scale induction, and explanations of their origins

From: Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry

Clone

Description

Δ1-708 BW30

Construct corresponding to Δ1-708 HaTRPA1 without GFP

Δ1-708 2.1.12

Clone 12 from plate 1 (low fluorescence)

Δ1-708 2.1.35

Clone 35 from plate 1 (average signal)

Δ1-708 1.2.1

Restreak 1 of clone 1.2b

Δ1-708 1.2.2a

Restreak 2 of clone 1.2b

Δ1-708 2.2.37

Clone 37 from plate 2 (similar to high control)

HaTRPA1 2.3.28

Clone 28 from plate 3 (low fluorescence)

HaTRPA1 2.3.12

Clone 12 from plate 3 (higher than high control)

HaTRPA1 1.2.1a

Restreak 1 of clone 1.2b

HaTRPA1 1.2.2

Restreak 2 of clone 1.2b

HaTRPA1 1.2.3

Restreak 3 of clone 1.2b

  1. aUsed for fed-batch expression
  2. bIsolated in the first screening round