Fig. 3

Batch cultivation of T. reesei with adapted medium. Scattered light and DO over time as well as pictures taken from the bottom and top of the cultivation plate at the end of cultivation for A wild type, B RutC30 and C RutC30 TR3158. Reproducibility of scattered light and DO for biological replicates was high for all strains in both media. However, cultures with adapted medium showed even higher reproducibility between biological replicates. Wall growth was comparably low for both media. Cultivation conditions: RWP, adapted medium, n = 1000 rpm, \({\text{d}_0}=3\) mm, 20 g l\(^{-1}\) glucose, \({\text{V}_\text{W}}=3.4\) ml, \({\text{V}_\text{L}}=1\) ml, humidity \(\ge 85\)%, \({\text{O}_{2}}=21\)%, T = 30 \(^\circ\)C, inoculum = 10\(^{5}\) spores ml\(^{-1}\), \({\text{n}_\text{bio}}=6\)